NDLI: Alleviation of oxygen stress on Neochloris oleoabundans: effects of bicarbonate and pH

Content Provider	Springer Nature Link
Author	Peng, Licheng Zhang, Zisheng Lan, Christopher Q. Basak, Ajoy Bond, Nicole Ding, Xiaohui Du, Jianjie
Copyright Year	2016
Abstract	This study investigated the deleterious effects of oxygen stress in a range of 100–400 % air saturation and verified the alleviation effects of the addition of bicarbonate and adjustment of culture pH. In the cultures with imposed dissolved oxygen (dO$_{2}$) stress at 400 % air saturation, the highest dry cell weight (DCW$_{max}$) of 1.04 ± 0.17 g L$^{−1}$, lipid content (LC) of 151.2 ± 4.8 mg g$^{−1}$ DCW and lipid productivity (LP) of 19.1 ± 0.8 mg L$^{−1}$ day$^{−1}$ were obtained in the cultures supplemented with 160 mM NaHCO$_{3}$, compared to a DCW$_{max}$ of 0.76 ± 0.018 g L$^{−1}$, LC of 116.3 ± 0.2 mg g$^{−1}$ DCW and LP of 9.96 ± 0.02 mg L$^{−1}$ day$^{−1}$ in the control (with 0 mM NaHCO$_{3}$). The results indicate that oxygen stress was hazardous to cell growth and impaired lipid accumulation in Neochloris oleoabundans. The addition of bicarbonate alleviated oxygen stress through increasing the ratio of dCO$_{2}$ to dO$_{2}$ to balance the photosynthesis and photorespiration, inhibiting the production of reactive oxygen species (ROS) and activating cellular antioxidant mechanisms such as enhanced lipids as an electron sink and ROS-scavenging antioxidant enzymes. Studies also show that the increase of pH in the range of 7.5–9.5 enhanced cell growth and lipid accumulation, although accompanied by increasing ROS concentration in cultures.
Starting Page	143
Ending Page	152
Page Count	10
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	29
Issue Number	1
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-08-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neochloris oleoabundans Chlorophyceae Oxygen stress Bicarbonate Non-sterile cultivation Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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