NDLI: Comparative evaluation of different preservation methods for cyanobacterial strains

Content Provider	Springer Nature Link
Author	Esteves Ferreira, Alberto A. Corrêa, Débora M. Carneiro, Antônio P. S. Rosa, Rinamara M. Loterio, Robson Araújo, Wagner L.
Copyright Year	2012
Abstract	Maintaining pure cultures using preservation methods is of high importance for biotechnological purposes. However, preservation does not necessarily guarantee the genetic stability of these cultures. Therefore, preservation methods are currently needed to assure viability as well as genetic, physiological, and morphological integrity across storage periods. In this study, preservation of five isolates from the microalgae and cyanobacteria collection of the Plant Biology Department, Federal University of Viçosa, Minas Gerais, Brazil was investigated via monthly analyses of cell viability, biomass recovery, and contaminant concentrations over a period of 120 days. Lyophilization was adequate for both heterocystous cyanobacteria and other strains that were able to differentiate hormogones or to synthesize thick layers of exopolysaccharides. Lyophilization was also able to maintain cultures with low levels of contaminants. Dimethyl sulfoxide was relatively efficient, though some of the strains were susceptible to its cytotoxic effects. Our results demonstrated that cryopreservation with glycerol was the most efficient method. The ability to routinely preserve cyanobacterial strains reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. The results obtained in this study are therefore discussed in the context of the efficiency of the methods and the current need to develop suitable methods for maintenance of cyanobacterial collections.
Starting Page	919
Ending Page	929
Page Count	11
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	25
Issue Number	4
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-10-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cyanobacteria Preservation methods Lyophilization Cryopreservation Cyanobacterial collections Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

From an environmental sample to a long-lasting culture: the steps to better isolate and preserve cyanobacterial strains

Evaluation of methanol preservation for molecular and morphological studies in cyanobacteria using Planktothrix agardhii

Impact of nitrogen and drought on phycobiliprotein content in terrestrial cyanobacterial strains

Biochemical characterization of some cyanobacterial strains from salt marshes of the Venice Lagoon

Characterization of exopolysaccharides produced by seven biofilm-forming cyanobacterial strains for biotechnological applications

The development of a cryopreservation method suitable for a large cyanobacteria collection

Emerging applications of cyanobacterial ultraviolet protecting compound scytonemin

Preservation methods of Tolypothrix tenuis for use as a cyanobacterial fertilizer

M13-based genotyping of marine cyanobacterial strains from the Indian subcontinent and maintained in the NFMC germplasm collection

Comparative evaluation of different preservation methods for cyanobacterial strains

Similar Documents

From an environmental sample to a long-lasting culture: the steps to better isolate and preserve cyanobacterial strains

Evaluation of methanol preservation for molecular and morphological studies in cyanobacteria using Planktothrix agardhii

Impact of nitrogen and drought on phycobiliprotein content in terrestrial cyanobacterial strains

Biochemical characterization of some cyanobacterial strains from salt marshes of the Venice Lagoon

Characterization of exopolysaccharides produced by seven biofilm-forming cyanobacterial strains for biotechnological applications

The development of a cryopreservation method suitable for a large cyanobacteria collection

Emerging applications of cyanobacterial ultraviolet protecting compound scytonemin

Preservation methods of Tolypothrix tenuis for use as a cyanobacterial fertilizer

M13-based genotyping of marine cyanobacterial strains from the Indian subcontinent and maintained in the NFMC germplasm collection

Comparative evaluation of different preservation methods for cyanobacterial strains