NDLI: Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

Content Provider	Springer Nature Link
Author	Kögel, Tanja Gerdes, Hans Hermann
Copyright Year	2010
Abstract	Neuroendocrine secretory granules (SGs) are formed at the trans-Golgi network (TGN) as immature intermediates. In PC12 cells, these immature SGs (ISGs) are transported within seconds to the cell cortex, where they move along actin filaments and complete maturation. This maturation process comprises acidification-dependent processing of cargo proteins, condensation of the SG matrix, and removal of membrane and proteins not destined to mature SGs (MSGs) into ISG-derived vesicles (IDVs). We investigated the roles of myosin Va and Rab3 isoforms in the maturation of ISGs in neuroendocrine PC12 cells. The expression of dominant-negative mutants of myosin Va or Rab3D blocked the removal of the endoprotease furin from ISGs. Furthermore, expression of mutant Rab3D, but not of mutant myosin Va, impaired cargo processing of SGs. In conclusion, our data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate in overlapping but not identical tasks.
Starting Page	1303
Ending Page	1308
Page Count	6
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	30
Issue Number	8
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2010-11-16
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Dense core vesicle Endocrine Exocytosis Furin Hormone Maturation Myosin Va Noc2 Processing Rab3 Rab27 Secretory granule Neurobiology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

REPORT Roles of Myosin Va and Rab3D in Membrane Remodeling

Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

Endocrine secretory granules and neuronal synaptic vesicles have three integral membrane proteins in common (1988).

Expression of Rab3D N135I inhibits regulated secretory ACTH in AtT-20 cells (1998)

Rab3D, a small GTPase, is localized on mast cell secretory granules and translocates to the plasma membrane upon exocytosis (1999)

Molecular mechanisms in exocytosis and endocytosis 199 versatile roles for myosin va in dense core vesicle biogenesis and function.

Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis

Involvement of the Rab27 binding protein Slac2c/MyRIP in insulin exocytosis (2003)

Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

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Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

REPORT Roles of Myosin Va and Rab3D in Membrane Remodeling

Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

Endocrine secretory granules and neuronal synaptic vesicles have three integral membrane proteins in common (1988).

Expression of Rab3D N135I inhibits regulated secretory ACTH in AtT-20 cells (1998)

Rab3D, a small GTPase, is localized on mast cell secretory granules and translocates to the plasma membrane upon exocytosis (1999)

Molecular mechanisms in exocytosis and endocytosis 199 versatile roles for myosin va in dense core vesicle biogenesis and function.

Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis

Involvement of the Rab27 binding protein Slac2c/MyRIP in insulin exocytosis (2003)

Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules