NDLI: Chiral Characterization and Quantification of Deprenyl-N-oxide and Other Deprenyl Metabolites in Rat Urine by Capillary Electrophoresis

Content Provider	Springer Nature Link
Author	Szöko, É. Tábi, T. Halász, A. S. Pálfi, M. Kalász, H. Magyar, K.
Copyright Year	2004
Abstract	Chiral capillary electrophoresis has been validated for the quantitative analysis of R-(-)-deprenyl (selegiline) and seven of its metabolites, among them the diastereomeric pair of selegiline-N-oxide in rat urine. Linear calibration curves were obtained over the concentration range: 0.5–100 μM for selegiline, N-desmethylselegiline, methamphetamine, amphetamine, and selegiline-N-oxides, and from 0.1-100 μM for para-hydroxylated derivatives of N-desmethylselegiline, methamphetamine, and amphetamine. The inter and intra-assay precision and accuracy varied by <15% for all analytes at concentrations of 2.5, 10 and 25 μM, and <20% at the lower limit of quantification (0.5 or 0.1 μM). The sample extraction procedure was optimised, and sample recoveries ranged: 80–111% and 74–91% at concentrations of 1 and 10 μM, respectively. The extracted urine samples retained quantitative accuracy for at least 5 days after storage at 4 °C. The validated method was used for in vivo metabolism studies in rats treated with either single or repeated dose of selegiline, or selegiline-N-oxide. Stereoselective N-oxidation of selegiline and rapid urinary excretion of selegiline-N-oxides have been observed. The most abundant metabolites of selegiline were the desalkylated and para-hydoxylated derivatives excreted in both conjugated and unconjugated forms in rat urine.
Starting Page	S245
Ending Page	S251
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	60
Issue Number	1
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2004-06-16
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis Method validation In vivo metabolism Deprenyl and deprenyl-N-oxide
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Achiral and chiral determination of tramadol and its metabolites in urine by capillary electrophoresis

Enantiomeric analysis of the five major monohydroxylated metabolites of methaqualone in human urine by chiral capillary electrophoresis

CE Determination of Ketoprofen Enantiomers in Clinical Samples of Plasma, Synovial Fluid and Urine

Separation of the enantiomers of four chiral drugs by neutral cyclodextrin-mediated capillary zone electrophoresis

Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis

Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis

Velocity-difference induced focusing of xanthine and purine metabolites by capillary electrophoresis using a dynamic pH junction

Validation and Further Optimisation of a Cyclodextrin-Modified Micellar Electrokinetic Capillary Chromatography Method for Urine Profiling

Chiral separation of deprenyl-N-oxide isomers by capillary electrophoresis using various cyclodextrin derivatives.

Chiral Characterization and Quantification of Deprenyl-N-oxide and Other Deprenyl Metabolites in Rat Urine by Capillary Electrophoresis

Similar Documents

Achiral and chiral determination of tramadol and its metabolites in urine by capillary electrophoresis

Enantiomeric analysis of the five major monohydroxylated metabolites of methaqualone in human urine by chiral capillary electrophoresis

CE Determination of Ketoprofen Enantiomers in Clinical Samples of Plasma, Synovial Fluid and Urine

Separation of the enantiomers of four chiral drugs by neutral cyclodextrin-mediated capillary zone electrophoresis

Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis

Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis

Velocity-difference induced focusing of xanthine and purine metabolites by capillary electrophoresis using a dynamic pH junction

Validation and Further Optimisation of a Cyclodextrin-Modified Micellar Electrokinetic Capillary Chromatography Method for Urine Profiling

Chiral separation of deprenyl-N-oxide isomers by capillary electrophoresis using various cyclodextrin derivatives.

Chiral Characterization and Quantification of Deprenyl-N-oxide and Other Deprenyl Metabolites in Rat Urine by Capillary Electrophoresis