NDLI: On the Reproducibility of Chromolith® RP Columns

Content Provider	Springer Nature Link
Author	Engelhardt, H. Götzinger, A.
Copyright Year	2004
Abstract	Monolithic columns are of growing importance in routine analysis. As all of these columns are solitaires, the reproducibility has been studied for the commercially available RP Chromolith column. The chromatographic test revealed that in respect of efficiency this column is equivalent a column (of identical length: 5 cm) packed with particles around 4 μm, however, the pressure drop corresponds to a column packed with particles around 10 μm. Per unit pressure drop about 6 times the plates are generated. Four Chromolith columns from different batches were compared in respect of efficiency and retention times. The plate numbers were 5330 with a RSD of 5%. The retention times varied less than 1.6%, corresponding to good batch to batch reproducibility as achieved in the preparation of bulk reversed phases. Because of the short column length and the relatively high linear velocities, data acquisition time has to be increased at least to 25 Hz to be able to fully exploid the potential efficiencies these columns are able to generate.
Starting Page	S207
Ending Page	S211
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	60
Issue Number	1
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2004-04-19
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Monolithic columns Pore size distribution Plates per unit pressure drop
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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