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Determination of bupivacaine and three of its metabolites in rat urine by capillary electrophoresis
| Content Provider | PubMed Central |
|---|---|
| Author | Schieferecke, Mark A. Mclaughlin, Kieran J. Faibushevich, Alexander A. Lunte, Craig E. |
| Abstract | A capillary electrophoretic (CE) method for the analysis of urinary extracts of the local anesthetic, bupivacaine, and its three main metabolites, desbutylbupivacaine, 3’-hydroxybupivacaine, and 4’-hydroxybupivacaine, in rat urine has been developed. The limits of detection were 0.22 μm for desbutylbupivacaine and bupivacaine, 0.15 μm for 3’-hydroxybupivacaine, and 0.16 μm for 4’-hydroxybupivacaine. The linear range was from 0.7 μm to 16.8 μm for all four compounds. Migration time and peak height reproducibilities, and extraction efficiencies were determined for all four compounds. Peak height reproducibilities (n = 5) for the overall method were improved through the use of prilocaine as an internal standard. Peak height reproducibilities were 5.6% RSD for desbutylbupivacaine and bupivacaine, and 9.9% RSD for 3’-hydroxybupivacaine and 4’-hydroxybupivacaine. Migration time reproducibilities (n = 5) were 2.4% for all compounds. Urine samples were collected from rats administered therapeutic doses of bupivacaine and extracted using a solid-phase extraction method (SPE). Separation of bupivacaine and its metabolites was achieved in 15 min. |
| Related Links | http://dx.doi.org/10.1002/elps.1150191633 |
| Ending Page | 3002 |
| Page Count | 6 |
| Starting Page | 2997 |
| File Format | |
| ISSN | 01730835 |
| Journal | Electrophoresis |
| Issue Number | 16-17 |
| Volume Number | 19 |
| Language | English |
| Publisher Date | 1998-11-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Biochemistry Biochemistry Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Clinical Biochemistry Biochemistry |
