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| Content Provider | Springer Nature Link |
|---|---|
| Author | Srbek, J. Bosáková, Z. Tesarová, E. Suchánková, J. Coufal, P. Němcová, I. |
| Copyright Year | 2004 |
| Abstract | Reversed-phase capillary liquid chromatography was applied for quantification of thioderivatives of acridine. Ten 9-(alkylthio)acridines, three containing and seven lacking amino groups, were analyzed on Nucleosil C18 with acetonitrile-water-triethylamine (90.0:9.0:1.0, v/v/v) mixture as the mobile phase. The presence of triethylamine in the eluent greatly improved the peak shape and markedly shortened the analysis time. Calibration curves of all the studied acridine derivatives were measured over a concentration range of 1.0·10−6 – 1.0·10−3 mol. L−1 at two parallel detection wavelengths, 265 and 270 nm. Detection and quantification limits were evaluated for all the derivatives. The detection limits were in the region of μmol. L−1 for most of the thioacridines. The calibration curves were used for quantification of the derivatives in human urine. No pretreatment of the real biological samples was necessary. The optimized CLC method enabled a good separation of the individual thioacridines from other compounds present in human urine samples and their quantification in a reasonable analysis time. |
| Starting Page | S37 |
| Ending Page | S41 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 60 |
| Issue Number | 1 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2004-02-26 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Capillary Columns 9-(Alkylthio)acridines Human urine samples |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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