NDLI: Homogeneous immunoassay for human IgG using oriented hen egg IgY immobilized on gold sol nanoparticles

Content Provider	Springer Nature Link
Author	Yeritsyan, Hermine E. Gasparyan, Vardan K.
Copyright Year	2011
Abstract	Homogeneous immunoassays using (red) gold nanoparticles represent an attractive detection scheme because of the option of photometric readout. We have applied oriented immobilization of hen egg immunoglobulin Y (IgY) on gold nanoparticles when developing a homogeneous immunoassay for human IgG. In oriented immobilization, as opposed to random immobilization, the antigen binding capabilities of the antibodies are retained. It is shown that such immunoassay has significantly better sensitivity in comparison with methods based on conventional immobilization of affinity-purified antibodies. It is also shown that hen egg IgY is better suited than rabbit antibodies, because much more antibody can be immobilized on gold nanoparticles without any destabilization, probably because of the more acidic nature of these antibodies. In addition, hen egg IgY can be supplied in higher quantity and can be prepared more easily than IgG from rabbits. Bleeding and slaughtering of animals is not needed. The assay presented here has a wide detection range (30–500 ng .mL−1) and a limit of detection as low as 30 ng.mL−1 of human IgG. Figure Nanoparticles are treated by thiol for formation of monolayer with exposed NH2 groups. IgY molecule is oxidized by periodate for formation of aldehyde group in Fc fragment. Consequent addition of such antibodies to gold nanoparticles results in binding of IgY molecules to gold nanoparticles via Fc fragment providing oriented immobilization.
Starting Page	117
Ending Page	122
Page Count	6
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	176
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2011-10-01
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gold nanoparticle Immunoassay Immunoglobulin Y Oriented immobilization Microengineering Characterization and Evaluation of Materials Nanotechnology Analytical Chemistry Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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