NDLI: Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detection

Content Provider	Springer Nature Link
Author	Yang, Xiaolan Pu, Jun Zhao, Hua Li, Xiaoyan Liao, Juan Xie, Yanling Zhu, Sha Long, Gaobo Yuan, Yonghua Liao, Fei
Copyright Year	2011
Abstract	A new screening method was tested to estimate the affinity of an aromatic ligand in a mixture through competitive binding against an exogenous reference ligand. The target protein was immobilized on magnetic particles and one or several ligand(s) in each reaction mixture were prepared by parallel combinatorial-synthesis without purification. Specifically, the binding of aromatic biotin derivatives to streptavidin immobilized on magnetic particles was used as the model. An approximation equation that works under the condition of binding ratios below 0.1 for the candidate ligand and the reference ligand was developed. It was found that the relative affinity of each biotinylated aromatic candidate ligand in mixtures was consistent with that by using HPLC-MS analyses or by a homogenous method using its purified counterpart. Hence, this new screening method using HPLC-UV is considered to be highly effective. Figure The representative procedure to realize the new screening technique for quantitative affinity of an aromatic candidate ligand of unknown quantity in a mixture sample
Starting Page	243
Ending Page	249
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	176
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2011-09-24
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Relative affinity HPLC-UV Magnetic separation Biotin derivatives Streptavidin Parallel-combinatorial-syntheses Microengineering Characterization and Evaluation of Materials Nanotechnology Analytical Chemistry Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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