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| Content Provider | Springer Nature Link |
|---|---|
| Author | Iqbal, Zafar Lai, Edward P. C. Avis, Tyler J. |
| Copyright Year | 2011 |
| Abstract | A rapid binding test has been developed for the detection of bacteria using polymer-modified magnetic nanoparticles. Polydopamine (PDA) can effectively act as a sorbent even in water solution, and a PDA coating on magnetic nanoparticles (MNPs) was therefore prepared to bind Escherichia coli (E. coli). Albeit non-selective, PDA-modified magnetic nanoparticles (MNPs@PDA) show nearly 100% efficiency in binding E. coli. If E. coli, grown in tryptic soy broth medium, is analyzed by capillary electrophoresis (CE) using phosphate buffer as the background electrolyte, two peaks are found, while a single peak is found with carbonate buffer containing 0.05% of poly(ethylene glycol). Self-polymerization of dopamine on E. coli at pH 9.5 is also feasible. The detection of E. coli is demonstrated by adding quantum dots (QDs) to form a QDs-PDA-E. coli aggregate for better CE analysis. Figure Development of Polymer-Modified Magnetic Nanoparticles and Quantum Dots for Escherichia coli Binding Test |
| Starting Page | 193 |
| Ending Page | 200 |
| Page Count | 8 |
| File Format | |
| ISSN | 00263672 |
| Journal | Microchimica Acta |
| Volume Number | 176 |
| Issue Number | 1-2 |
| e-ISSN | 14365073 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2011-10-16 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Escherichia coli Polydopamine-coated magnetic nanoparticles Quantum dots Rapid binding test Capillary electrophoresis Microengineering Characterization and Evaluation of Materials Nanotechnology Analytical Chemistry Nanochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry |
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