NDLI: L-Argininamide biosensor based on S1 nuclease hydrolysis signal amplification

Content Provider	Springer Nature Link
Author	Hun, Xu Wang, Zhouping
Copyright Year	2011
Abstract	A sensitive method is presented for the detection of L-argininamide. It is based on the amplification of the hydrolysis of S1 nuclease of single-stranded regions of an aptamer-target complex. The S1 nuclease, which is sequence-independent, is used to “recycle” target molecules, thus leading to strongly enhanced chemiluminescence (CL). L-Argininamide was chosen as model analyte. The DNA aptamer and its complementary DNA were labeled with the CL reagent N-(4-aminobutyl)-N-ethylisoluminol (ABEI). The DNA complementary to the aptamer was labeled with ABEI and immobilized on magnetic beads (MBs) coated with gold. The aptamer was also labeled with ABEI and self-assembled on the MBs. A duplex was formed due to hybridization between the DNA aptamer and the DNA complementary to the aptamer. In the presence of the target L-argininamide, a stem-loop aptamer structure is formed which subsequently denatures the duplex. This switch from a duplex structure to a stem-loop structure causes the formation of single-stranded regions both in the target-aptamer and in the single-stranded DNA on the MBs. The nuclease hydrolyzes the single-stranded regions and single-stranded DNA. Ultimately, L-argininamide is released which then interacts with another aptamer on the MB, thereby leading to one more L-argininamide. This autocatalytic cycle can generate substantial quantities of ABEI which then can be sensitively determined by the diperiodatonickelate-isoniazide reaction system. L-argininamide can be detected in the concentration range from 3.0 × 10−4 to 3.0 × 10−7 M, and the limit of detection is 1.0 × 10−7 M. Figure A enantiomer assay for detection of L-argininamide was developed based on S1 nuclease hydrolysis of single-stranded regions of aptamer-target complex and the releasing of the L-argininamide. The released L-argininamide can then interact with another aptamer leading to many signal probes be generated. The L-argininamide assay exhibits high sensitivity and specificity.
Starting Page	209
Ending Page	216
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	176
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2011-10-21
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	S1 nuclease L-argininamide Cycle Signal amplification Microengineering Characterization and Evaluation of Materials Nanotechnology Analytical Chemistry Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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