NDLI: A SERS-based immunoassay for porcine circovirus type 2 using multi-branched gold nanoparticles

Content Provider	Springer Nature Link
Author	Luo, Zhihui Li, Wentao Lu, Donglian Chen, Kun He, Qigai Han, Heyou Zou, Mingqiang
Copyright Year	2013
Abstract	We report on a facile immunoassay for porcine circovirus type 2 (PCV2) based on surface enhanced Raman scattering (SERS) using multi-branched gold nanoparticles (mb-AuNPs) as substrates. The mb-AuNPs in the immunosensor act as Raman reporters and were prepared via Tris base-induced reduction and subsequent reaction with p-mercaptobenzoic acid (pMBA). They possess good stability and high SERS activity. Subsequently, the modified mb-AuNPs were covalently conjugated to the monoclonal antibody (McAb) against the PCV2 cap protein to form SERS immuno nanoprobes. These were captured in a microtiterplate via a immunoreaction in the presence of target antigens. The effects of antibody concentration, reaction time and temperature on the sensitivity of the immunoassay were investigated. Under optimized assay conditions, the Raman signal intensity at 1,076 cm−1 increases logarithmically with the concentrations of PCV2 in the concentration ranging from 8 × 102 to 8 × 106 copies per mL. The limit of detection is 8 × 102 copies per mL. Compared to conventional detecting methods such as those based on PCR, the method presented here is rapid, facile and very sensitive. Figure A simple and novel approach to detect porcine circovirus type 2 using surface enhanced Raman scattering (SERS) of multi-branched gold nanoparticles is demonstrated, it has a higher sensitivity than polymerase chain reaction and ELISA.
Starting Page	1501
Ending Page	1507
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	15-16
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-08-06
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Porcine circovirus type 2 Immunoassay Surface enhanced Raman scattering Multi-branched gold nanoparticles Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Synthesis of multi-branched gold nanoparticles by reduction of tetrachloroauric acid with Tris base, and their application to SERS and cellular imaging

Fabrication of SERS active gold nanorods using benzalkonium chloride, and their application to an immunoassay for potato virus X

Surface-enhanced Raman scattering detection of bacteria on microarrays at single cell levels using silver nanoparticles

Gold nanoparticle based optical and electrochemical sensing of dopamine

Silver–gold core-shell nanoparticles containing methylene blue as SERS labels for probing and imaging of live cells

A Surface Enhanced Raman Scattering (SERS) microdroplet detector for trace levels of crystal violet

Photochemical decoration of gold nanoparticles on polymer stabilized magnetic microspheres for determination of adenine by surface-enhanced Raman spectroscopy

Highly sensitive SERS probe for mercury(II) using cyclodextrin-protected silver nanoparticles functionalized with methimazole

Continuous flow synthesis and characterization of tailor-made bare gold nanoparticles for use in SERS

A SERS-based immunoassay for porcine circovirus type 2 using multi-branched gold nanoparticles

Similar Documents

Synthesis of multi-branched gold nanoparticles by reduction of tetrachloroauric acid with Tris base, and their application to SERS and cellular imaging

Fabrication of SERS active gold nanorods using benzalkonium chloride, and their application to an immunoassay for potato virus X

Surface-enhanced Raman scattering detection of bacteria on microarrays at single cell levels using silver nanoparticles

Gold nanoparticle based optical and electrochemical sensing of dopamine

Silver–gold core-shell nanoparticles containing methylene blue as SERS labels for probing and imaging of live cells

A Surface Enhanced Raman Scattering (SERS) microdroplet detector for trace levels of crystal violet

Photochemical decoration of gold nanoparticles on polymer stabilized magnetic microspheres for determination of adenine by surface-enhanced Raman spectroscopy

Highly sensitive SERS probe for mercury(II) using cyclodextrin-protected silver nanoparticles functionalized with methimazole

Continuous flow synthesis and characterization of tailor-made bare gold nanoparticles for use in SERS

A SERS-based immunoassay for porcine circovirus type 2 using multi-branched gold nanoparticles