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| Content Provider | Springer Nature Link |
|---|---|
| Author | Poboży, Ewa Król, Edyta Wójcik, Lena Wachowicz, Mariusz Trojawicz, Marek |
| Copyright Year | 2010 |
| Abstract | Perfluorinated carboxylic acids (PFCAs) represent an important group of persistent perfluorinated organic compounds commonly determined in environmental and biological samples. A reversed-phase HPLC method was developed based on derivatization of the PFCAs with the commercially available fluorescent reagent 3-bromoacetyl coumarin. The method was optimized and this resulted in the efficient separation of PFCAs containing from 3 to 12 carbon atoms in molecule in 25 min run. To improve sensitivity, the preconcentration step has been optimized using Oasis-WAX and C18 sorbents for SPE. A 100-fold preconcentration is achieved by solid-phase extraction with the sorbent C18 Sep-PAK to result in limits of detection in the range from 43 to 75 ppt for the analytes examined, and in the application of the method of water analysis. Figure Chromatogram of mixture of perfluorinated carboxylic acids C3-PFCA – C12-PFCA with fluorescence detection after derivatization with 3-bromoacetyl coumarin (b), and blank (a) |
| Starting Page | 409 |
| Ending Page | 417 |
| Page Count | 9 |
| File Format | |
| ISSN | 00263672 |
| Journal | Microchimica Acta |
| Volume Number | 172 |
| Issue Number | 3-4 |
| e-ISSN | 14365073 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2010-12-12 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Perfluorinated carboxylic acids HPLC Fluorescence detection Analytical Chemistry Nanotechnology Nanochemistry Microengineering Characterization and Evaluation of Materials |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry |
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