NDLI: Cynomolgus macaque (Macaca fascicularis) immunoglobulin heavy chain locus description

Content Provider	Springer Nature Link
Author	Yu, Guo Yun Mate, Suzanne Garcia, Karla Ward, Michael D. Brueggemann, Ernst Hall, Matthew Kenny, Tara Sanchez Lockhart, Maria Lefranc, Marie Paule Palacios, Gustavo
Copyright Year	2016
Abstract	Cynomolgus macaques (Macaca fascicularis) have become an important animal model for biomedical research. In particular, it is the animal model of choice for the development of vaccine candidates associated with emerging dangerous pathogens. Despite their increasing importance as animal models, the cynomolgus macaque genome is not fully characterized, hindering molecular studies for this model. More importantly, the lack of knowledge about the immunoglobulin (IG) locus organization directly impacts the analysis of the humoral response in cynomolgus macaques. Recent advances in next generation sequencing (NGS) technologies to analyze IG repertoires open the opportunity to deeply characterize the humoral immune response. However, the IG locus organization for the animal is required to completely dissect IG repertoires. Here, we describe the localization and organization of the rearranging IG heavy (IGH) genes on chromosome 7 of the cynomolgus macaque draft genome. Our annotation comprises 108 functional genes which include 63 variable (IGHV), 38 diversity (IGHD), and 7 joining (IGHJ) genes. For validation, we provide RNA transcript data for most of the IGHV genes and all of the annotated IGHJ genes, as well as proteomic data to validate IGH constant genes. The description and annotation of the rearranging IGH genes for the cynomolgus macaques will significantly facilitate scientific research. This is particularly relevant to dissect the immune response during vaccination or infection with dangerous pathogens such as Ebola, Marburg and other emerging pathogens where non-human primate models play a significant role for countermeasure development.
Starting Page	417
Ending Page	428
Page Count	12
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	68
Issue Number	6-7
e-ISSN	14321211
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-05-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Macaca fascicularis Cynomolgus macaque Variable genes Diversity genes Joining genes V-D-J rearrangement Immunology Human Genetics Gene Function Cell Biology Allergology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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