Simultaneous determination of seventeen glucocorticoids residues in milk and eggs by ultra-performance liquid chromatography/electrospray tandem mass spectrometry.

Content Provider	Semantic Scholar
Author	Cui, Xiaoliang Shao, Bing Zhao, Rong Yang, Yi Hu, Jianying Tu, Xiaoming
Copyright Year	2006
Abstract	A comprehensive analytical method has been developed and validated for the simultaneous determination of seventeen glucocorticoid residues in eggs and milk. The mass spectrometer parameters, the composition of the mobile phase and the sample preparation method were firstly optimized to obtain maximum sensitivity. The samples were deconjugated with beta-glucuronidase/arylsulfatase enzyme and concentrated using an Oasis HLB solid-phase extraction cartridge, followed by cleanup with a dual Sep-pak silica and aminopropyl cartridge. The analytes were quantified by ultra-performance liquid chromatography (using a C18 column)/electrospray ionization tandem mass spectrometry (UPLC/ESI-MS/MS) operating in the negative ion mode. The assay for the 17 glucocorticoids was linear over the range of 1-200 microg/L for milk and egg samples with a high correlation coefficient (>0.99). The limits of quantification (LOQs) for the target analytes were 0.04-1.27 microg/kg for the egg samples and 0.03-0.73 microg/kg for the milk samples. The average extraction recoveries of the glucocorticoids from eggs and milk at two concentration levels (spiked at 0.40 and 2.00 microg/kg) were 65.6-118.7% and 61.5-119.6%, respectively, with relative standard deviations between 1.8-17.0% and 2.4-18.4%, respectively. Because of its high sensitivity, good precision and specificity, the method was found to be suitable for trace analysis of synthetic and natural glucocorticoids in complex biosamples such as eggs and milk.
Starting Page	1
Ending Page	5
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ues.pku.edu.cn/szdw/personal/hjy/download/RCMS-2006-20.pdf
PubMed reference number	16835849v1
Volume Number	20
Issue Number	15
Journal	Rapid communications in mass spectrometry : RCM
Language	English
Access Restriction	Open
Subject Keyword	Arylsulfatases Coefficient Concentrate Dosage Form Drug abuse Dual Egg Food Product Eluent Ions Limit of Detection Liquid Chromatography Mass Spectrometers (device) Milk (body substance) Milk, Human Natural Science Disciplines Quantitation Recovery - action Silicic Acid Tandem Mass Spectrometry analyte beta Thalassemia
Content Type	Text
Resource Type	Article