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Time- and concentration-dependent cytotoxicity of antibiotics used in endodontic therapy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ferreira, Marina Beloti Myiagi, Suely Nogales, Carlos Goes Campos, Márcia Sampaio Lage-Marques, José Luiz |
| Copyright Year | 2010 |
| Abstract | OBJECTIVE New drugs have to be assessed in endodontic therapy due to the presence of microorganisms resistant to therapeutic procedures. Thus, this study evaluated the time- and concentration-dependent cytotoxicity of different antibiotics used in endodontic therapy. MATERIAL AND METHODS Human gingival fibroblasts were treated and divided into the following experimental groups: Group I - control; Group II - ciprofloxacin hydrochloride; Group III - clyndamicin hydrochloride; and Group IV - metronidazole. Each drug was used at concentrations of 5, 50, 150, and 300 mg/L for 24, 48, 72, and 96 h. Cytotoxicity was evaluated by the MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and spectrophotometric reading of ELISA plates. The results were analyzed by BioEstat 4.0 software using Kruskal-Wallis and Dunn's tests at a significance level of 5%. Cell viability was assessed for the different concentrations and times. RESULTS All drugs presented dose-dependent cytotoxicity. Concentrations of 5 and 50 mg/L produced viable fibroblasts at all experimental times in all groups. CONCLUSIONS Cell viability at 24 h was greater than in the other experimental times. Comparison between the same concentrations of antibiotics at different times showed that metronidazole presented the highest cell viability at 72 and 96 h compared to the other antibiotics, whereas clyndamicin hydrochloride showed higher cell viability at 72 h than ciprofloxacin hydrochloride. |
| Starting Page | 259 |
| Ending Page | 263 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.scielo.br/pdf/jaos/v18n3/v18n3a11.pdf |
| PubMed reference number | 20857004v1 |
| Volume Number | 18 |
| Journal | Journal of applied oral science : revista FOB |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Survival Cellular Phone Ciprofloxacin Hydrochloride Enzyme-Linked Immunosorbent Assay Gingiva Leukemia, B-Cell Metronidazole Microorganism Therapeutic procedure methscopolamine bromide monooxyethylene trimethylolpropane tristearate significance level |
| Content Type | Text |
| Resource Type | Article |
