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In vitro evaluation of acute cytotoxicity of human chemically treated allografts.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dufrane, Denis Cornu, Olivier H. Verraes, T. Schecroun, N. Banse, Xavier Schneider, Yves Delloye, C. |
| Copyright Year | 2001 |
| Abstract | In order to minimize the risk of contamination associated with tissue transplantation, tissue banks commonly chemically treat the tissues whenever possible. As viral inactivation uses agents lethal to microorganisms, it is imperative to assure that chemically inactivated tissue remains biocompatible. In vitro assays can be an effective means to assess the acute cytotoxicity of chemically treated human allografts. We have used different types of cells cultured in the presence of treated tissue extract. A standard cell line, a human fibroblast (WI38), which was the same for all the samples, was chosen. In addition, as the banked tissues (bone and fascia lata) were prepared to be used in bone or as a dura mater substitute, two other cell types were also used: an osteoblastic cell line (SaOS-2) and a neuronal cell line (Neuro 2A). Cytotoxic assessment was performed by qualitative evaluation of cell morphology based on confluence, granulation, vacuolization and swelling analysis. In addition, quantitative methods based on the use of neutral red (NR) and 3- (4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) were assayed. Qualitative and quantitative evaluation of fascia lata and bone extracts did not show deleterious effects on cell cultures. These results show that in vitro methods can be appropriate to select a non-toxic procedure before it is used in the human body and that several strong chemical treatments can result in a tissue suitable for human. |
| Starting Page | 664 |
| Ending Page | 664 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://dial.uclouvain.be/downloader/downloader.php?datastream=PDF_01&disclaimer=59e07c8e9caca306f05617a9dce8f2826883376f0bb5a5b3a33e9346ed3607c9&pid=boreal:10634 |
| Alternate Webpage(s) | http://www.ecmjournal.org/journal/papers/vol001/pdf/v001a06 |
| PubMed reference number | 16821194v1 |
| Volume Number | 1 |
| Journal | European cells & materials |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Allograft surgical material Bank (environment) Body tissue Bone Tissue Cell Culture Techniques Cellular Morphology Cultured Cell Line Dura Mater Edema Fascia lata structure Granulation procedure Microorganism Neutral Red Tetrazolium Salts Tissue Banks Tissue Transplantation Transplantation, Homologous Virus Diseases methscopolamine bromide monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
