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MicroRNA-340 Inhibits Tumor Cell Proliferation and Induces Apoptosis in Endometrial Carcinoma Cell Line RL 95-2
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xie, Wei Qin, Wen Kang, Yalin Zhou, Ziyan Qin, Aiping |
| Copyright Year | 2016 |
| Abstract | BACKGROUND The purpose of our study was to investigate the functional role of microRNA-340 (miR-340) in endometrial carcinoma (EC). MATERIAL AND METHODS Human EC cell line RL 95-2 was transfected with miR-340 mimics, inhibitors, or controls. After 48 h of transfection, the cell viability was determined by 3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl -2-H-tetrazolium bromide (MTT) assay. The BrdU assay and apoptosis assay were performed to determine the effects of miR-340 mimics or inhibitors on cell proliferation and apoptosis, respectively. The underlying mechanisms involved in cell proliferation and apoptosis were explored by measuring the protein levels of cell cycle regulators (p27 kinase inhibition protein (KIP) 1 and p21) and apoptosis-related factors (B-cell lymphoma-2 (Bcl-2), Bax, pro-Caspase 3, and active-Caspase-3). RESULTS Overexpression of miR-340 significantly inhibited the cell viability (P<0.05) and cell proliferation (P<0.01) of RL 95-2 cells compared with the control group, but increased the apoptosis (P<0.01). However, suppression of miR-340 had opposite results. Moreover, the protein levels of p27 KIP1, Bax, pro-Caspase 3, and active-Caspase-3 were significantly increased by overexpression of miR-340 but were statistically decreased by suppression of miR-340. Contrary results were found in the protein levels of Bcl-2. However, no significant differences were found in p21 expression. CONCLUSIONS MiRNA-340 acts as an anti-oncogene in EC cell line RL 95-2 by inhibition of tumor cell proliferation and induction of apoptosis. |
| Starting Page | 1540 |
| Ending Page | 1546 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 27153225v1 |
| Volume Number | 22 |
| Journal | Medical science monitor : international medical journal of experimental and clinical research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | BCL2 gene Bromodeoxyuridine Cell Cycle Cell Proliferation Cell Survival Conflict (Psychology) Endometrial Carcinoma Leukemia, B-Cell Neoplasms Oncogenes Positive Regulation of Apoptosis RELN gene RELN wt Allele Tetrazolium Salts Tumor Suppressor Genes caspase-3 interest methscopolamine bromide monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
