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Experimental hyperphenylalaninemia provokes oxidative stress in rat brain.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hagen, Martine E. Kienzle Pederzolli, Carolina Didonet Sgaravatti, Angela Malysz Bridi, Raquel Wajner, Moacir Wannmacher, Clóvis Milton Duval Wyse, Angela T. S. Dutra-Filho, Carlos Severo |
| Copyright Year | 2002 |
| Abstract | Tissue accumulation of L-phenylalanine (Phe) is the biochemical hallmark of human phenylketonuria (PKU), an inherited metabolic disorder clinically characterized by mental retardation and other neurological features. The mechanisms of brain damage observed in this disorder are poorly understood. In the present study we investigated some oxidative stress parameters in the brain of rats with experimental hyperphenylalaninemia. Chemiluminescence, total radical-trapping antioxidant potential (TRAP), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were measured in the brain of the animals. We observed that chemiluminescence is increased and TRAP is reduced in the brain of hyperphenylalaninemic rats. Similar data were obtained in the in vitro experiments using Phe at various concentrations. CAT activity was significantly inhibited by Phe in vitro and in vivo, whereas GSH-Px activity was reduced in vivo but not in vitro and SOD activity was not altered by any treatment. The results indicate that oxidative stress may be involved in the neuropathology of PKU. However, further studies are necessary to confirm and extend our findings to the human condition and also to determine whether an antioxidant therapy may be of benefit to these patients. |
| Starting Page | 326 |
| Ending Page | 352 |
| Page Count | 27 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82316682.pdf |
| PubMed reference number | 11997085v1 |
| Volume Number | 1586 |
| Issue Number | 3 |
| Journal | Biochimica et biophysica acta |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Antioxidants Body tissue Brain Injuries CATALASE Chemiluminescence Classical phenylketonuria GLUTATHIONE PEROXIDASE Hyperphenylalaninaemia In Vitro [Publication Type] Mental Retardation Metabolic Diseases Oxidative Stress Patients Phenylalanine Phenylketonuria, Maternal Superoxide Dismutase Superoxides neuropathology disease |
| Content Type | Text |
| Resource Type | Article |
