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Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines
| Content Provider | Semantic Scholar |
|---|---|
| Author | Isnansetyo, Alim Lutfia, Fadilah Nor Laili Nursid, Muhammad Trijoko Susidarti, Ratna Asmah Fadilah Lutfia, Nor Laili Nursid, Mad |
| Copyright Year | 2016 |
| Abstract | Introduction: Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT (3-[4,5-dimethylthiazol-2-yl] -2,5diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC50 ranging between 461-663 μg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed by fucoidan from Padina sp. with IC50 of 144 and 118 μg/mL, respectively. While its IC50 against Vero cells was 501 μg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC50 of 60, 63 and 211 μg/mL against MCF-7, WiDr and Vero Cells, respectively. Although the IC50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 μg/mL. Conclusion: These results indicated that Padina sp.fucoidan showed potential selective cytotoxicity, and promising for the development of an anti-cancer compound. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.phcogj.com/sites/default/files/10.5530pj.2017.1.3.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 2-diethylaminoethanol Algae Cellulose Colon Carcinoma Colon classification Column Chromatography DEAE-Cellulose Fucus Information extraction Inhibitory Concentration 50 Neoplasms Phytophthora inflata Polysaccharides Purify Spectrometry Turbinaria |
| Content Type | Text |
| Resource Type | Article |
