NDLI: Cooperative Binding of the Organophosphate Paraoxon to the (Na+ + K+)-ATPase

Please wait, while we are loading the content...

Cooperative Binding of the Organophosphate Paraoxon to the (Na+ + K+)-ATPase

Content Provider	Scilit
Author	Blasiak, Janusz
Copyright Year	1995
Abstract	Paraoxon. the main active metabolite of the organophosphorus insecticide parathion. exerted a dose-dependent inhibitory effect on the activity of pig kidney (Na⁺ + K⁺)-ATPase contained in microsomal fraction and purified from it. Substrate kinetics studies revealed the existence of two active sites with high and low affinity to ATP. The Dixon analysis of the mode of the inhibition indicated its noncompetitive character. The purified enzyme was more affected than enzyme contained in the microsomal fraction. The inhibition constant K, ranged from 73 to 246 μm depending on the type of preparation. The Hill coefficient (n) fulfilled the relationship 1<n<3. These properties of the interaction suggest the cooperative binding of paraoxon to the enzyme. An indirect mechanism of the interaction was proposed: paraoxon could inhibit the activity of the (N a⁺ + K⁺)-ATPase by excluding the enzyme protein from its normal lipid milieu
Related Links	http://www.degruyter.com/downloadpdf/j/znc.1995.50.issue-9-10/znc-1995-9-1010/znc-1995-9-1010.xml
Ending Page	663
Page Count	4
Starting Page	660
e-ISSN	18657125
DOI	10.1515/znc-1995-9-1010
Journal	Zeitschrift für Naturforschung C
Issue Number	9-10
Volume Number	50
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1995-10-01
Access Restriction	Open
Subject Keyword	Zeitschrift Für Naturforschung C Toxicology Organophosphorus Insecticide. Insecticide-membrane Interaction. Parathion. (na+ + K+)-atpase Lipid-protein Interaction Journal: Zeitschrift für Naturforschung C, Vol- 50
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparison of the Action of an Organophosphorus Insecticide and Its Metabolite on Chloride and Sulfate Transport in Erythrocyte Membrane

Altered Fatty Acid, Cholesterol and Na+/K+ ATPase Activity in Erythrocyte Membrane of Rheumatoid Arthritis Patients

Permeability of Plasma Membrane Vesicles to Ouabain and Mg2+ as a Factor Determining Rate of Binding of Ouabain to Na+ and K+ Dependent ATPase

Effect of Aging on the Activities of Acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase in Rat Pituitary and Hypothalamus

ʟ-Phenylalanine Effect on Rat Diaphragm Acetylcholinesterase and Na+,K+-ATPase

Effect of CDP-choline on Hippocampal Acetylcholinesterase and $Na^{+},K^{+}$-ATPase in Adult and Aged Rats

The Membrane ATPase of the (Mg, Ca)-ATPase-Mutant 12, strain AN 120

Ouabain-Insensitive $Na^{+}$-ATPase Activity in Trypanosoma cruzi Epimastigotes

The Use of Fluorescent Probes for Studying the Interaction of Proteins with Black Lipid Membranes

Cooperative Binding of the Organophosphate Paraoxon to the (Na+ + K+)-ATPase

Similar Documents

Comparison of the Action of an Organophosphorus Insecticide and Its Metabolite on Chloride and Sulfate Transport in Erythrocyte Membrane

Altered Fatty Acid, Cholesterol and Na+/K+ ATPase Activity in Erythrocyte Membrane of Rheumatoid Arthritis Patients

Permeability of Plasma Membrane Vesicles to Ouabain and Mg2+ as a Factor Determining Rate of Binding of Ouabain to Na+ and K+ Dependent ATPase

Effect of Aging on the Activities of Acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase in Rat Pituitary and Hypothalamus

ʟ-Phenylalanine Effect on Rat Diaphragm Acetylcholinesterase and Na+,K+-ATPase

Effect of CDP-choline on Hippocampal Acetylcholinesterase and $Na^{+},K^{+}$-ATPase in Adult and Aged Rats

The Membrane ATPase of the (Mg, Ca)-ATPase-Mutant 12, strain AN 120

Ouabain-Insensitive $Na^{+}$-ATPase Activity in Trypanosoma cruzi Epimastigotes

The Use of Fluorescent Probes for Studying the Interaction of Proteins with Black Lipid Membranes

Cooperative Binding of the Organophosphate Paraoxon to the (Na+ + K+)-ATPase