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Ouabain-Insensitive $Na^{+}$-ATPase Activity in Trypanosoma cruzi Epimastigotes

Content Provider	Scilit
Author	Caruso-Neves, Celso Einicker-Lamas, Marcelo Chagas, Carlos Oliveira, Mecia Maria Vieyra, Adalberto Lopes, Aníbal Gil
Copyright Year	1999
Abstract	In the present paper, the presence of a ouabain-insensitive Na⁺-stimulated, Mg²⁺-dependent ATPase activity in T. cruzi epimastigotes CL14 clone and Y strain was investigated. The increase in Na⁺ concentration (from 5 to 170 mᴍ), in the presence of 2 mᴍ ouabain, increases the ATPase activity in a saturable manner along a rectangular hyperbola. The was 18.0 ± 1.0 and 21.1 ± 1.1 nmoles Pi x mg^-1 x min^-1 and the half-activation value (K₅₀) for Na⁺ was 34.3 ± 5.8 mᴍ and 37.7 ± 5.3 in CL14 clone and in Y strain, respectively. The Na⁺-stimulated ATPase activity was inhibited by 5-[aminosulfonyl]-4-chloro-2-[(2-furanylmethyl)-amino] benzoic acid (furosemide) in a dose-dependent manner. The half-inhibition value (I₅₀) was 0.22 ± 0.03 and 0.24 ± 0.07 mᴍ, and the Hill number (n) was 0.99 ± 0.2 and 2.16 ± 0.29 for CL14 clone and Y strain, respectively. These data indicate that both cell types express the ouabain-insensitive Na⁺-ATPase activity, which might be considered the biochemical expression of the second Na⁺ pump
Related Links	http://www.degruyter.com/downloadpdf/j/znc.1999.54.issue-1-2/znc-1999-1-217/znc-1999-1-217.xml
Ending Page	104
Page Count	5
Starting Page	100
e-ISSN	18657125
DOI	10.1515/znc-1999-1-217
Journal	Zeitschrift für Naturforschung C
Issue Number	1-2
Volume Number	54
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1999-02-01
Access Restriction	Open
Subject Keyword	Zeitschrift Für Naturforschung C Pharmacology and Pharmacy Na+-atpase T. Cruzi Atpase Epimastigote Furosemide Journal: Zeitschrift für Naturforschung C, Vol- 54
Content Type	Text
Resource Type	Article

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