NDLI: Permeability of Plasma Membrane Vesicles to Ouabain and Mg2+ as a Factor Determining Rate of Binding of Ouabain to Na+ and K+ Dependent ATPase

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Permeability of Plasma Membrane Vesicles to Ouabain and Mg2+ as a Factor Determining Rate of Binding of Ouabain to Na+ and K+ Dependent ATPase

Content Provider	Scilit
Author	Walter, Horst
Copyright Year	1979
Abstract	Na⁺, K⁺-ATPase of the plasma membrane isolated from sheep kidney medulla exhibits functional asymmetry for the cardiac glycoside ouabain. In this vesicular membrane preparation the rate of binding of ouabain was slow (time constant > 60 min) when the vesicles were incubated in the presence of isotonic sucrose. Upon treatment of the preparation with hypoosmotic shock or phospholipase A the initial rate of ouabain binding was enhanced at least 3 fold. In equilibrium a concentration of the ouabain-enzyme-complex was obtained which was about twofold that of the untreated vesicles. This result suggests two types of ouabain binding sites with an approximate stoichiometry of 1 to 1. The stoichiometry seems to be maintained at high concentrations of ouabain where binding curves show a biphasic time course. Additional information about heterogeneity of binding sites comes through experiments in which the vesicles were treated with Mg²⁺prior to the addition of ouabain. A minor fraction of the binding sites were occupied by ouabain only after longtime incubation with Mg²⁺.
Related Links	http://www.degruyter.com/downloadpdf/j/znc.1979.34.issue-12/znc-1979-1225/znc-1979-1225.xml
Ending Page	1231
Page Count	8
Starting Page	1224
e-ISSN	18657125
DOI	10.1515/znc-1979-1225
Journal	Zeitschrift für Naturforschung C
Issue Number	12
Volume Number	34
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1979-12-01
Access Restriction	Open
Subject Keyword	Zeitschrift Für Naturforschung C Biochemistry and Molecular Biology Na+ K+-atpase Plasma Membrane Vesicles Cardiac Glycoside Magnesium Tightness Journal: Zeitschrift für Naturforschung C, Issue- 190
Content Type	Text
Resource Type	Article

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