NDLI: F-Actin reassembly during focal adhesion impacts single cell mechanics and nanoscale membrane structure

Content Provider	Springer Nature Link
Author	Zimmer, Christopher C. Shi, LiFang Shih, YiPing Li, JieRen Jin, LeeWay Lo, SuHao Liu, GangYu
Copyright Year	2012
Abstract	Focal adhesions play an important role in cell spreading, migration, and overall mechanical integrity. The relationship of cell structural and mechanical properties was investigated in the context of focal adhesion processes. Combined atomic force microscopy (AFM) and laser scanning confocal microscopy (LSCM) was utilized to measure single cell mechanics, in correlation with cellular morphology and membrane structures at a nanometer scale. Characteristic stages of focal adhesion were verified via confocal fluorescent studies, which confirmed three representative F-actin assemblies, actin dot, filaments network, and long and aligned fibrous bundles at cytoskeleton. Force-deformation profiles of living cells were measured at the single cell level, and displayed as a function of height deformation, relative height deformation and relative volume deformation. As focal adhesion progresses, single cell compression profiles indicate that both membrane and cytoskeleton stiffen, while spreading increases especially from focal complex to focal adhesion. Correspondingly, AFM imaging reveals morphological geometries of spherical cap, spreading with polygon boundaries, and elongated or polarized spreading. Membrane features are dominated by protrusions of 41–207 nm tall, short rods with 1–6 μm in length and 10.2–80.0 nm in height, and long fibrous features of 31–246 nm tall, respectively. The protrusion is attributed to local membrane folding, and the rod and fibrous features are consistent with bilayer decorating over the F-actin assemblies. Taken collectively, the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics, spreading morphology, and membrane structural features.
Starting Page	1922
Ending Page	1930
Page Count	9
File Format	PDF
ISSN	16747291
Journal	Science in China Series B: Chemistry
Volume Number	55
Issue Number	9
e-ISSN	18691870
Language	English
Publisher	SP Science China Press
Publisher Date	2012-03-16
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	focal adhesion actin fibers actin assembly fibroblast membrane structure single cell mechanics atomic force microscopy laser scanning confocal microscopy Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry

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