NDLI: Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)

Content Provider	Royal Society of Chemistry (RSC)
Author	Chung, Hee-Chung Kwon, Yong-Kook Jang, Zi-Hey Ahn, Yun Gyong Kim, Cheol-Hee Kim, Jin-Sup Ryu, Jae-Ho Ryu, Do Hyun Hwang, Geum-Sook
Copyright Year	2012
Abstract	Zebrafish (Danio rerio) is becoming a popular developmental biology model to study diseases and for drug discovery. In this study, we performed proton nuclear magnetic resonance spectroscopy (1H-NMR)- and gas chromatography–mass spectrometry (GC/MS)-based metabolic profiling of an alcoholic fatty liver using a zebrafish disease model. We examined metabolic differences between the control and alcoholic fatty liver groups in zebrafish to determine how metabolism in an alcoholic fatty liver is regulated. Multivariate statistical analysis showed a significant difference between the control and alcoholic fatty liver groups. The alcoholic fatty liver group showed increased excretion of isoleucine, acetate, succinate, choline, creatine, acetoacetate, 3-hydroxybutyrate (3HB), ethyl glucuronide (EtG), lactate/pyruvate ratio, fatty acids, and cholesterol, and decreased excretion of citrate, aspartate, tyrosine, glycine, glucose, alanine, betaine, and maltose. Metabolites identified in the fatty liver groups were associated with long-term alcohol consumption, which causes both oxidation–reduction (redox) changes and oxidative stress. This study suggests that global metabolite profiling in a zebrafish model can provide insights into the metabolic changes in an alcoholic fatty liver.
Starting Page	2001
Ending Page	2009
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	7
Journal	Molecular BioSystems
DOI	10.1039/c2mb25073j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxidative stress Metabolite Ethyl glucuronide Pyruvic acid Proton nuclear magnetic resonance Glucose Creatine Glycine Betaine Acetoacetic acid Isoleucine Tyrosine Gas chromatography\u2013mass spectrometry Alanine Redox Zebrafish Nuclear magnetic resonance spectroscopy Maltose Metabolism Cholesterol Citric acid Choline Succinic acid Danio Drug discovery Lactic acid Molecular biology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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