NDLI: A multi-analytical approach for metabolomic profiling of zebrafish (Danio rerio) livers

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Contents and Chemical Biology

Protein allostery, signal transmission and dynamics: a classification scheme of allosteric mechanisms

High throughput methods of assessing protein stability and aggregation

High-density peptide arrays

Homoadenine signalling system for SNP typing

Human telomeric DNA sequences have a peroxidase apoenzyme activity

A photostable fluorescent probe for targeted imaging of tumour cells possessing integrin α_vβ₃**

RNA targeting through binding of small molecules: Studies on t-RNA binding by the cytotoxic protoberberine alkaloid coralyne

Modularity and robustness of bone networks

Development of a multiplexed bead-based assay for detection of DNA methylation in cancer-related genes

HPC2 and ubinuclein define a novel family of histone chaperones conserved throughout eukaryotes

Dynamic network topology changes in functional modules predict responses to oxidative stress in yeast

A multi-analytical approach for metabolomic profiling of zebrafish (Danio rerio) livers

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Molecular BioSystems Vol - 5 Issue - 2

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A multi-analytical approach for metabolomic profiling of zebrafish (Danio rerio) livers

Content Provider	Royal Society of Chemistry (RSC)
Author	Zou, Li Chor, Cui Fang Ong, Choon Nam Ong, Eng Shi
Copyright Year	2009
Abstract	A metabolomic study was performed to investigate the biochemical profiles of livers from male and female zebrafish (Danio rerio), using a multiple platform approach, incorporating 1H NMR, GC/MS and LC/MS. The reproducibility and reliability of the three methods were validated prior to the assays. Major biomolecules detected using one method were also cross examined using the other techniques. These metabolites included carbohydrates, lipids, amino acids detected using 1H NMR and GC/MS, and acetylcarnitine, choline and various phospholipids determined using 1H NMR and LC/MS. Our findings suggest that 1H NMR provided comprehensive information on glucose, amino acids, pyruvate and other smaller biochemical constituents of the zebrafish liver. On the other hand, GC/MS was able to assay cholesterol, saturated and unsaturated fatty acids, and LC/MS was ideal for the analysis of lipids/phospholipids. These techniques revealed that there are significant differences in the biochemical profiles of male and female zebrafish liver tissue extracts. Specifically, we noted that although there were no significant differences observed for the carbohydrate profile, the amino acid profile was rather different in male and female zebrafish liver. Furthermore, data from all three techniques revealed that although the saturated fatty acid profile was similar, the compositions of unsaturated fatty acids were different in the two phenotypes. The overall findings suggested that this multiplatform approach offers comprehensive coverage of a metabolome as well as provides valuable insight towards understanding the different biochemical profiles of a biosystem.
Starting Page	288
Ending Page	298
Page Count	11
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	5
Issue Number	3
Journal	Molecular BioSystems
DOI	10.1039/b811850g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Liver Metabolome Zebrafish Pyruvic acid Glucose Cholesterol Acetylcarnitine Choline Fatty acid Carbohydrate Danio Nuclear magnetic resonance
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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A multi-analytical approach for metabolomic profiling of zebrafish (Danio rerio) livers

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