NDLI: Metabolomics-on-a-chip and metabolic flux analysis for label-free modeling of the internal metabolism of HepG2/C3A cells

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Molecular BioSystems Vol - 8 Issue - 6

Molecular BioSystems Vol - 8 Issue - 5

Molecular BioSystems Vol - 8 Issue - 4

Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Metabolomics-on-a-chip and metabolic flux analysis for label-free modeling of the internal metabolism of HepG2/C3A cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Bunescu, Andrei Brochot, Céline Ouattara, Djomangan Adama Prot, Jean-Matthieu Dumas, Marc-Emmanuel Elena-Herrmann, Bénédicte Leclerc, Eric
Copyright Year	2012
Abstract	In vitro microfluidic systems are increasingly used as an alternative to standard Petri dishes in bioengineering and metabolomic investigations, as they are expected to provide cellular environments close to the in vivo conditions. In this work, we combined the recently developed “metabolomics-on-a-chip” approach with metabolic flux analysis to model the metabolic network of the hepatoma HepG2/C3A cell line and to infer the distribution of intracellular metabolic fluxes in standard Petri dishes and microfluidic biochips. A high pyruvate reduction to lactate was observed in both systems, suggesting that the cells operate in oxygen-limited environments. Our results also indicate that HepG2/C3A cells in the biochip are characterized by a higher consumption rate of oxygen, presumably due to a higher oxygenation rate in the microfluidic environment. This leads to a higher entry of the ultimate glycolytic product, acetyl-CoA, into the Krebs cycle. These findings are supported by the transcriptional activity of HepG2/C3A cells in both systems since we observed that genes regulated by a HIF-1 (hypoxia-regulated factor-1) transcriptional factor were over expressed under the Petri conditions, but to a lesser extent in the biochip.
Starting Page	1908
Ending Page	1920
Page Count	13
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	7
Journal	Molecular BioSystems
DOI	10.1039/c2mb25049g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Oxygen Metabolic network Metabolic flux analysis Krebs Pyruvic acid Citric acid cycle Lactic acid Acetyl-CoA Biological engineering
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Multiple Mass Isotopomer Tracing of Acetyl-CoA Metabolism in Langendorff-perfused Rat Hearts CHANNELING OF ACETYL-CoA FROM PYRUVATE DEHYDROGENASE TO CARNITINE ACETYLTRANSFERASE

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