NDLI: A gold nanoparticles based immuno-bioprobe for detection of Vi capsular polysaccharide of Salmonella enterica serovar Typhi

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Molecular BioSystems Vol - 8 Issue - 6

Molecular BioSystems Vol - 8 Issue - 5

Molecular BioSystems Vol - 8 Issue - 4

Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

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A gold nanoparticles based immuno-bioprobe for detection of Vi capsular polysaccharide of Salmonella enterica serovar Typhi

Content Provider	Royal Society of Chemistry (RSC)
Author	Tiwari, R. P. Pandey, Satish Kumar Suri, C. Raman Rishi, Praveen Chaudhry, M.
Copyright Year	2012
Abstract	A rapid and sensitive gold-nanobioprobe based immunoassay format has been presented for the detection of capsular Vi polysaccharide of Salmonella enterica serovar Typhi (surface antigen) using anti-Vi antibodies. The Vi antigen was extracted from serovar Typhi cells, under the optimised growth conditions for its over-expression. Anti-Vi antibodies were produced and conjugated with gold nanoparticles (GNPs) of definite size (∼30 nm), which served as the nano-bioprobe in the detection system. A sandwich immunoassay was developed using nitrocellulose dot blot comb (8/12 wells) membranes immobilized with anti-Salmonella antibodies at the optimal concentration (43 ng spot−1). The Vi antigen in the clinical isolates, spiked samples and also in the standard strain (serovar Typhi Ty2) was detected by measuring the colour intensity of GNPs and correlating it with the concentration of serovar Typhi in samples. Using this developed immunoassay technique Vi positive serovar Typhi strains could be detected with a sensitivity of up to 102 cells mL−1 in the clinical isolates as well as in the spiked samples. The developed immunoassay technique could be useful for the detection of typhoid fever and may be important from an epidemiological point of view.
Starting Page	1853
Ending Page	1860
Page Count	8
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	7
Journal	Molecular BioSystems
DOI	10.1039/c2mb25048a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Antigen Dot blot Immunoassay Antibody Typhoid fever Serotype Polysaccharide Nitrocellulose
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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