NDLI: Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE

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Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE

Content Provider	Royal Society of Chemistry (RSC)
Author	Sarto, Cecilia Salemi, Claudia Pitto, Marina Morosi, Lavinia Bianchi, Cristina Perego, Roberto Fumagalli, Daniela Brambilla, Paolo Magni, Fulvio Raimondo, Francesca Chinello, Clizia Ferrero, Stefano Rocco, Francesco
Copyright Year	2012
Abstract	Renal cell carcinoma (RCC), the most common neoplasm affecting the adult kidney, is characterised by heterogeneity of histological subtypes, drug resistance, and absence of molecular markers. Two-dimensional difference gel electrophoresis (2-D DIGE) technology in combination with mass spectrometry (MS) was applied to detect differentially expressed proteins in 20 pairs of RCC tissues and matched adjacent normal kidney cortex (ANK), in order to search for RCC markers. After gel analysis by DeCyder 6.5 and EDA software, differentially expressed protein spots were excised from Deep Purple stained preparative 2DE gel. A total of 100 proteins were identified by MS out of 2500 spots, 23 and 77 of these were, respectively, over- and down-expressed in RCC. The Principal Component Analysis applied to gels and protein spots exactly separated the two sample classes in two groups: RCC and ANK. Moreover, some spots, including ANXA2, PPIA, FABP7 and LEG1, resulted highly differential. The DIGE data were also confirmed by immunoblotting analysis for these proteins. In conclusion, we suggest that applying 2-D DIGE to RCC may provide the basis for a better molecular characterization and for the discovery of candidate biomarkers.
Starting Page	1040
Ending Page	1051
Page Count	12
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	4
Journal	Molecular BioSystems
DOI	10.1039/c2mb05390j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Western blot Difference gel electrophoresis Protein RCC Renal cell carcinoma FABP7 Electronic design automation Annexin A2 PPIA Mass spectrometry Renal cortex Deep Purple Principal component analysis
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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