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Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Differential protein expression in tears of patients with primary open angle and pseudoexfoliative glaucoma

Content Provider	Royal Society of Chemistry (RSC)
Author	Agnifili, Luca Mastropasqua, Alessandra Sacchetta, Paolo Ilio, Carmine Di Ciancaglini, Marco Fasanella, Vincenzo D'Aguanno, Simona Urbani, Andrea Mastropasqua, Leonardo Pieragostino, Damiana Bucci, Sonia Boccio, Piero Del
Copyright Year	2012
Abstract	Primary open angle (POAG) and pseudoexfoliative glaucoma (PXG) are the most common primary and secondary forms of glaucoma, respectively. Even though the patho-physiology, aqueous humor composition, risk factors, clinical features, therapy and drug induced ocular surface changes in POAG and PXG have been widely studied, to date information concerning tear protein characterization is lacking. Tears are a source of nourishment for ocular surface tissues and a vehicle to remove local waste products, metabolized drugs and inflammatory mediators produced in several ophthalmic diseases. In glaucoma, the proteomic definition of tears may provide insights concerning patho-physiology of the disease and ocular surface modifications induced by topical therapy. Our study aimed at characterizing protein patterns in tears of patients with medically controlled POAG and PXG. A comparative tears proteomic analysis by label-free LC-MSE highlighted differences in the expression of several proteins in the two glaucoma sub-types and control subjects, highlighting inflammation pathways expressed in both diseases. Results were independently reconfirmed by SDS-PAGE and linear MALDI-TOF MS, validating altered levels of Lysozyme C, Lipocalin-1, Protein S100, Immunoglobulins and Prolactin Inducible Protein. Moreover, we found a differential pattern of phosphorylated Cystatin-S that distinguishes the two pathologies. The most relevant results suggest that in both pathologies there may be active inflammation pathways related to the disease and/or induced by therapy. We show, for the first time, tear protein patterns expressed under controlled intraocular pressure conditions in POAG and PXG subjects. These findings could help in the understanding of molecular machinery underlying these ophthalmologic diseases, resulting in early diagnosis and more specific therapy.
Starting Page	1017
Ending Page	1028
Page Count	12
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	4
Journal	Molecular BioSystems
DOI	10.1039/c1mb05357d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	POAG Glaucoma Polyacrylamide gel electrophoresis Aqueous humour Inflammation Route of administration Protein S100 protein Prolactin Inducible Protein Ionization Prolactin Lysozyme Intraocular pressure
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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