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Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

A proteomic profile of washing fluid from the colorectal tract to search for potential biomarkers of colon cancer

Content Provider	Royal Society of Chemistry (RSC)
Author	Torregrossa, Liborio Iacconi, Pietro Chiarugi, Massimo Proietti, Agnese Basolo, Fulvio Ciregia, Federica Giannaccini, Gino Donadio, Elena Giusti, Laura Valle, Ylenia Da Ventroni, Tiziana Lucacchini, Antonio
Copyright Year	2012
Abstract	Washing fluid (WF) from the colon rectal tract after surgical resection might represent a first step in obtaining a mixture of proteins derived from the secretion of tumoral epithelial cells potentially involved in the pathological progression of tissue. In this study, we performed a proteomic analysis of colorectal WF to search for potential biomarkers of colon cancer. The outcome of this approach might open the possibility of using WF to screen for the precancerous and early stages of colorectal cancer (CRC). Samples of WFs were obtained during surgery from 35 patients submitted to colon resection for suspicious adenocarcinoma or carcinoma, while the respective controls were obtained by washing the healthy sections. WFs were immediately centrifuged, concentrated and trichloroacetic acid (TCA) was added to obtain protein pellets. After two-dimensional gel electrophoresis (2DE), the protein patterns of malignant samples were compared with respective normal samples. Forty-one protein spots were found to be differentially expressed exhibiting ≥2 fold-change of mean value spot intensities. After mass spectrometry, these protein spots collapsed into 38 different proteins. Interestingly, 19 of the differentially expressed proteins identified in the study corresponded to those suggested as being potential biomarkers of CRC. In accordance with the literature, these proteins showed the same direction of change (up or down for all proteins). Our results suggest that WF has the potential of being a method for the exploration of clinical samples for biomarker and drug target discovery.
Starting Page	1088
Ending Page	1099
Page Count	12
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	4
Journal	Molecular BioSystems
DOI	10.1039/c2mb05394b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Adenocarcinoma Biomarker TCA Carcinoma CRC Colorectal cancer Rectum Two-dimensional gel electrophoresis Mass spectrometry Protein
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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