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Proteomic analysis of climatic keratopathy droplets.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Menegay, Michelle Lee, DeMia Tabbara, Khalid F. Cafaro, Thamara A. Urrets-Zavalía, Julio Alberto Serra, Horacio M. Bhattacharya, Sanjoy K. |
| Copyright Year | 2008 |
| Abstract | PURPOSE To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation. |
| Starting Page | 193 |
| Ending Page | 199 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://iovs.arvojournals.org/pdfaccess.ashx?url=/data/journals/iovs/932952/z7g00708002829.pdf |
| PubMed reference number | 18378572v1 |
| Alternate Webpage(s) | https://doi.org/10.1167/iovs.07-1438 |
| DOI | 10.1167/iovs.07-1438 |
| Journal | Investigative ophthalmology & visual science |
| Volume Number | 49 |
| Issue Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Annexin A2 Body tissue Encyclopedias Fibril - cell component GAPDH gene Genome Glyceraldehyde Patients Polyacrylamide Gel Electrophoresis Specimen Spectrometry Subgroup bullous keratopathy cyclin-dependent protein kinase activity |
| Content Type | Text |
| Resource Type | Article |
