NDLI: A biocompatible micro cell culture chamber (µCCC) for the culturing and on-line monitoring of eukaryote cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Stangegaard, Michael Christensen, C. B. V. Jørgensen, A. M. Dufva, M. Petronis, S.
Copyright Year	2006
Abstract	We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.
Starting Page	1045
Ending Page	1051
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	8
Journal	Lab on a Chip
DOI	10.1039/b603379b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Microscope Cell culture Gene Perfusion Para-Methoxy-N-methylamphetamine
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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