NDLI: Survival, migration and differentiation of retinal progenitor cells transplanted on micro-machined poly(methyl methacrylate) scaffolds to the subretinal space

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Survival, migration and differentiation of retinal progenitor cells transplanted on micro-machined poly(methyl methacrylate) scaffolds to the subretinal space

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Back matter

Back cover

Lab on a Chip Vol - 7 Issue - 5

Lab on a Chip Vol - 7 Issue - 4

Lab on a Chip Vol - 7 Issue - 3

Lab on a Chip Vol - 7 Issue - 2

Survival, migration and differentiation of retinal progenitor cells transplanted on micro-machined poly(methyl methacrylate) scaffolds to the subretinal space

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhang, Yiqin Tao, Sarah Klassen, Henry Redenti, Stephen Desai, Tejal Young, Michael J. Young, Conan
Copyright Year	2007
Abstract	Stem and progenitor cells can be combined with polymer substrates to generate tissue equivalents in culture. The replacement of retinal tissue lost to disease or trauma using retinal progenitor cells (RPCs) delivered on polymer scaffolds and transplanted into the sub-retinal space of the damaged retina is a promising therapeutic strategy. Micromachining-based, ultra-thin PMMA poly(methyl methacrylate) scaffolds may provide a suitable cytoarchitectural environment for tissue engineering and transplantation to the diseased eye. Here, adhesion of RPCs to polymer, as well as migration and differentiation in the host retina were compared for PMMA scaffolds (6 µm thickness) with either smooth or porous (11 µm diameter) surface topography. RPCs were cultured under identical conditions on smooth or porous laminin-coated polymer scaffolds and transplanted into the subretinal space of C57BL/6 mice. RPCs could be cultured on both scaffolds with similar results, although transplantation with non-porous scaffolds showed limited RPC retention. Porous scaffolds demonstrated enhanced RPC adherence during transplantation and allowed for greater process outgrowth and cell migration into the host retinal layers. Integrated cells expressed the mature neuronal marker neurofilament-200 (nf-200), the glial marker glial fibrillary acidic protein (GFAP) and the retinal-specific marker recoverin. No host foreign body response was seen. In conclusion, ultra-thin film PMMA scaffolds micromachined to contain through pores retain adherent RPCs to a considerably greater extent than unmachined versions during the transplantation process and can serve as a biocompatible substrate for cell delivery in vivo.
Starting Page	695
Ending Page	701
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	6
Journal	Lab on a Chip
DOI	10.1039/b618583e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	RPC Tissue engineering Glial fibrillary acidic protein RPCs Polymer Retina Poly(methyl methacrylate) Recoverin Para-Methoxy-N-methylamphetamine Neurofilament Micrometre Biocompatibility
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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