NDLI: Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

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Research Highlights ( Lab on a Chip Vol - 6 Issue - 8 )

Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

Microchannel device using self-spreading lipid bilayer as molecule carrier

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection

Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screens

Nanofabrication of electrode arrays by electron-beam and nanoimprint lithographies

Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose

High-efficiency electrokinetic micromixing through symmetric sequential injection and expansion

Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform

A biocompatible micro cell culture chamber (µCCC) for the culturing and on-line monitoring of eukaryote cells

Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with electrochemical detection

Application of a temperature-controllable microreactor to simple and rapid protein identification using MALDI-TOF MS

On-chip syringe pumps for picoliter-scale liquid manipulation

Initial study of two-phase laminar flow extraction chip for sample preparation for gas chromatography

High-performance flow-focusing geometry for spontaneous generation of monodispersed droplets

Low melting point agarose as a protection layer in photolithographic patterning of aligned binary proteins

Stable electric-field driven cone-jetting of concentrated biosuspensions

Photopolymerized check valve and its integration into a pneumatic pumping system for biocompatible sample delivery

Compact fluorescence detection using in-fiber microchannels—its potential for lab-on-a-chip applications

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Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

Content Provider	Royal Society of Chemistry (RSC)
Author	Park, Min Cheol Hur, Jae Young Park, Sang-Hyun Suh, Kahp Y. Kwon, Keon Woo
Copyright Year	2006
Abstract	We present a simple cell docking method induced by receding meniscus to capture non-adherent yeast cells onto microwells inside a microfluidic channel. Microwells were fabricated either by capillary moulding of UV curable polyurethane acrylate (PUA) onto glass substrate or direct replica moulding of poly(dimethyl siloxane) (PDMS). A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into the microfluidic channel by surface tension driven capillary flow and a receding meniscus was subsequently generated by evaporation. As the meniscus progressed, one to multiple yeast cells were spontaneously captured onto microwells by lateral capillary force created at the bottom of the meniscus. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (α-factor) by monitoring the expression of green fluorescent protein (GFP) with time. It was observed that α-factor triggered the expression of GFP at 60 min after stimulation and the fluorescence intensity was sustained for an additional 60 min without changes.
Starting Page	988
Ending Page	994
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	8
Journal	Lab on a Chip
DOI	10.1039/b602961b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Yeast Simple cell Fluorescence Pheromone GFP Capillary action Acrylate polymer Polyurethane Siloxane Green fluorescent protein Surface tension Seduction community Saccharomyces cerevisiae
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Receding meniscus induced docking of yeast cells inside microfluidic channels at single cell level

A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes

Single-Cell Level Array of Yeast Cells in Pumpless Microfluidic Channels induced by Receding Meniscus

A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes

Fluorescence polarisation of green fluorescent protein (GFP). A strategy for improved wavelength discrimination for GFP determinations

GFP-inspired fluorescent polymer

Systematic analysis of S. cerevisiae chromosome VIII genes

Coplanar electrode microfluidic chip enabling accurate sheathless impedance cytometry

A pumpless microfluidic device driven by surface tension for pancreatic islet analysis

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

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