NDLI: An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models

Content Provider	Royal Society of Chemistry (RSC)
Author	Yamamoto, Takatoki Fujii, Teruo Kimura, Hiroshi Sakai, Yasuyuki Sakai, Hitomi
Copyright Year	2008
Abstract	Conventional cell-based assays in life science and medical applications can be difficult to maintain functionally over long periods. Microfluidics is an emerging technology with potential to provide integrated environments for cell maintenance, continuous perfusion, and monitoring. In this study, we developed an integrated microfluidic device with on-chip pumping and detection functionalities. The microfluidic structure in the device is divided into two independent channels separated by a semipermeable membrane on which cells are inoculated and cultured. Perfusion and fluorescence measurements of culture media for each channel can be conducted by the on-chip pumping system and optical fiber detection system. Performance of the device was examined through long-term culture and monitoring of polarized transport activity of intestinal tissue models (Caco-2 cells). The cells could be cultured for more than two weeks, and monolayer transport of rhodamine 123 was successfully monitored by on-line fluorescent measurement. This device may have applications in toxicity testing and drug screening.
Starting Page	741
Ending Page	746
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/b717091b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Rhodamine Semipermeable membrane Perfusion Caco-2 Fluorescence The Cells Microfluidics Optical fiber
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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