NDLI: Targetable and fixable rotor for quantifying mitochondrial viscosity of living cells by fluorescence lifetime imaging

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Journal of Materials Chemistry B Vol -4

Journal of Materials Chemistry B Vol -3

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Targetable and fixable rotor for quantifying mitochondrial viscosity of living cells by fluorescence lifetime imaging

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Ning Song, Xinbo Wang, Chao Xiao, Yi
Copyright Year	2017
Abstract	It is meaningful to accurately quantify the changes in local viscosity within the mitochondria of living cells, because viscosity influences mitochondrial network organization and metabolite diffusion. Although many molecular probes targeting mitochondria have been reported, almost all of them are not fixed to the mitochondria. Thus, they may not be suitable for sensing in abnormal mitochondria with lowered potential. In order to monitor viscosity in all mitochondria, no matter their working or health status, we develop the first fixable BODIPY (boron-dipyrromethene) rotor, named Vis-A. Vis-A contains an aldehyde group as an anchor to react with amino groups of proteins, which make it immobilizable in mitochondria. Vis-B, the reference compound without such anchor unit, is also synthesized. Both Vis-A and Vis-Bshow excellent mitochondrial targetability, as good as the commercially available mitochondrial dye (Mito Tracker Deep Red). However, when cells are chemically treated to decrease the mitochondrial potential, only Vis-A continues emitting strong fluorescence in mitochondria, but the signals of Vis-B and Mito Tracker Deep Red completely disappear. This comparison confirms that Vis-A not only specifically localizes in mitochondria, but also can be stably retained there. Then, Vis-A is applied to detect the mitochondrial viscosity of living cells by Fluorescence Lifetime Imaging (FLIM). Especially, with the aid of Vis-A, the changes in viscosity under typical pathological conditions (i.e., treatment with rotenone and carbonylcyanide-m-chlorophenylhydrazone (CCCP)) for mitochondria are monitored by FLIM.
Starting Page	360
Ending Page	368
Page Count	9
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	5
Issue Number	2
Journal	Journal of Materials Chemistry B
DOI	10.1039/c6tb02524b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Mito Tracker Deep Red FLIM Viscosity Mitochondrion Metabolite Diffusion Molecular Probes BODIPY Aldehyde Staining Mito Domain Red Fluorescence Rotenone
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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