NDLI: Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors

Content Provider	Semantic Scholar
Author	Steinmark, Ida Emilie James, Arjuna L. Dreiss, Cécile A. Yahioglu, Gokhan Suhling, Klaus
Copyright Year	2019
Abstract	Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.
File Format	PDF HTM / HTML
DOI	10.1117/12.2508676
Volume Number	10893
Alternate Webpage(s)	https://kclpure.kcl.ac.uk/portal/files/113374675/PhotWest_Emilie_final.pdf
Alternate Webpage(s)	https://doi.org/10.1117/12.2508676
Journal	BiOS
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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