NDLI: Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates <i>via</i> liquid-phase laser ablation and their enhanced osteogenic differentiation activity

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Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity

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Journal of Materials Chemistry B Vol -4

Journal of Materials Chemistry B Vol -3

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Facile fabrication of bioactive ultra-small protein–hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity

Content Provider	Royal Society of Chemistry (RSC)
Author	Rodio, Marina Romeo, Elisa Intartaglia, Romuald Coluccino, Luca Garau, Gianpiero Diaspro, Alberto Genovese, Alessandro
Copyright Year	2017
Abstract	Hydroxyapatite bioactive complexes are being increasingly recognized as effective available means in regenerative medicine. Conventional technologies for their synthesis have drawbacks from a synthetic standpoint, mainly requiring high temperatures and multi-step processes. Here, we show that ultra-small hydroxyapatite conjugated-nanoparticles (Ha-CNPs) can be obtained at room temperature by Pulsed Laser Ablation (PLA) directly in protein solution using picosecond pulses at near infrared wavelengths. The results showed that the nanoparticle size was driven by the concentration of the protein. Using this approach, we obtained aqueous soluble and ultra-small crystalline nanoparticles of ≈3 nm diameter coated with protein molecules (surface coverage ≈ 5.5 pmol cm−2; zeta potential ≈−33.5 mV). These nanoparticles showed low cytotoxicity in vitro compared to chemically synthesized nanoparticles, and revealed proliferative and osteoinductive effects on human bone marrow mesenchymal stem cells (hMSCs). The resulting enhanced cell osteogenic differentiation suggested that our PLA-based synthetic approach might be exploited in novel applications of regenerative medicine.
Starting Page	279
Ending Page	288
Page Count	10
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	5
Issue Number	2
Journal	Journal of Materials Chemistry B
DOI	10.1039/c6tb02023b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Pulsed Laser Ablation PLA Hydroxylapatite Regenerative medicine Protein Picosecond Infrared spectroscopy Nanoparticle Zeta potential Cytotoxicity Bone marrow Mesenchymal stem cell
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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