NDLI: The apoptosis of OVCAR-3 induced by TNF-α plus IFN-γ co-immobilized polylactic acid copolymers

Content Provider	Royal Society of Chemistry (RSC)
Author	Zheng, Zhe Liu, Jun-Ming Li, Zhibin Guan, Yan-Qing Liang, Lihua Zhang, Lin Du, Jinju
Copyright Year	2012
Abstract	The cell cycle arrest and apoptosis of human ovarian cancer OVCAR-3 cells, induced by three kinds of co-immobilized polylactic acid copolymer materials, compared with the corresponding control groups and free groups, are investigated in vitro. The three kinds of polylactic acid copolymers are PLGA (poly(lactic-co-glycolic acid)), PDLLA (poly(DL-lactic acid)), and PEG-PLGA (polyethylene glycol-poly(lactic-co-glycolic acid) copolymers). They are respectively photo-immobilized with TNF-α plus IFN-γ as the co-immobilized group, respectively mixed with TNF-α plus IFN-γ as the free group. First, the co-immobilized groups are prepared and examined by Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR), atomic force microscopy (AFM), and electron spectroscopy for chemical analysis (ESCA). Second, the cell morphology, cell mortality, and cell cycle arrest of OVCAR-3 cells induced by the co-immobilized group are analyzed by ematoxylin staining and flow cytometry. Third, western immunoblot analysis of the protein expression of p53, Bax, and Bcl-2 and immunofluorescence analysis of the protein location of caspase-3 in the induced OVCAR-3 cells were also performed. The results show that the co-immobilized group shows more visible inhibition activity towards OVCAR-3 cells than the control group and free group, after a time of 48 hours and at a concentration level of 20 ng ml−1. The OVCAR-3 cells mediated by the co-immobilized group are individually arrested in the G1, G2, and S phases. Moreover, the western blotting and immunofluorescence analysis reveals that the treatment of OVCAR-3 cells by the co-immobilized group remarkably enhances the cellular expression of activated p53, Bax, and caspase-3, suggesting that the mitochondrial pathway of apoptosis may be significant. Our data thus provide the microscopic surface characterization and cell biology basis for utilizing the TNF-α plus IFN-γ co-immobilized polylactic acid copolymers as potential antitumorigenic targeted drugs.
Starting Page	14746
Ending Page	14755
Page Count	10
File Format	HTM / HTML PDF
ISSN	09599428
Volume Number	22
Issue Number	29
Journal	Journal of Materials Chemistry
DOI	10.1039/c2jm31972a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	PLGA ESCA Fourier Bax Cell cycle Apoptosis Ovarian cancer Acid Copolymer Polylactic acid Polyethylene Tumor necrosis factor alpha Interferon gamma Free group Fourier transform Infrared spectroscopy Atomic-force microscopy Electron spectroscopy Flow cytometry Protein P53 Bcl-2-associated X protein Bcl-2 Immunofluorescence Caspase Western blot Molecular biology
Content Type	Text
Resource Type	Article
Subject	Chemistry Materials Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway

Increased p53 level, bax/bcl-x l ratio, and trail receptor expression in human emphysema.

Dietary High Fluorine Induces Apoptosis and Alters Bcl-2, Bax, and Caspase-3 Protein Expression in the Cecal Tonsil Lymphocytes of Broilers

Grape Seed Proanthocyanidins Induce Apoptosis through p53, Bax, and Caspase 3 Pathways

G-00150-2005.r1 role of mitochondria in aspirin-induced apoptosis in human gastric.

Mitigation of apoptosis-mediated neurotoxicity induced by silver nanoparticles via rutaceae nutraceuticals: P53 activation and Bax/Bcl-2 regulation.

Ginger polysaccharide UGP1 suppressed human colon cancer growth via p53, Bax/Bcl-2, caspase-3 pathways and immunomodulation

The relationship between cisplatin-induced apoptosis and p53, bcl-2 and bax expression in human lung cancer cells.

Interferon- - induces apoptosis of lens tn4–1 cells and proteasome inhibition has an antiapoptotic effect.

The apoptosis of OVCAR-3 induced by TNF-α plus IFN-γ co-immobilized polylactic acid copolymers

Similar Documents

Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway

Increased p53 level, bax/bcl-x l ratio, and trail receptor expression in human emphysema.

Dietary High Fluorine Induces Apoptosis and Alters Bcl-2, Bax, and Caspase-3 Protein Expression in the Cecal Tonsil Lymphocytes of Broilers

Grape Seed Proanthocyanidins Induce Apoptosis through p53, Bax, and Caspase 3 Pathways

G-00150-2005.r1 role of mitochondria in aspirin-induced apoptosis in human gastric.

Mitigation of apoptosis-mediated neurotoxicity induced by silver nanoparticles via rutaceae nutraceuticals: P53 activation and Bax/Bcl-2 regulation.

Ginger polysaccharide UGP1 suppressed human colon cancer growth via p53, Bax/Bcl-2, caspase-3 pathways and immunomodulation

The relationship between cisplatin-induced apoptosis and p53, bcl-2 and bax expression in human lung cancer cells.

Interferon- - induces apoptosis of lens tn4–1 cells and proteasome inhibition has an antiapoptotic effect.

The apoptosis of OVCAR-3 induced by TNF-α plus IFN-γ co-immobilized polylactic acid copolymers