The relationship between cisplatin-induced apoptosis and p53, bcl-2 and bax expression in human lung cancer cells.

Content Provider	Europe PMC
Author	Han, Ji-Youn Chung, Yeun-Jun Park, Sung Won Kim, Jung Soo Rhyu, Mun-Gan Kim, Hoon-Kyo Lee, Kyung Shick
Copyright Year	1999
Abstract	ObjectivesGiven the roles of bcl-2, bax and p53 in apoptosis, we investigated the effect of their expression on the response to cisplatin in order to understand the molecular events of cisplatin-resistance in lung cancers.MethodsThree parental human lung cancer cell lines (PC9, PC14 and H69) and their in vitro selected cisplatin-resistant sublines were examined. Cells treated with cisplatin were processed for acridine orange and ethidium bromide staining and DNA gel electrophoresis for the morphologic detection of apoptosis. The endogenous levels of bcl-2, bax and p53 protein expression in lung cancer cells were assessed by Western blot analysis and DNA of polymerase chain reaction-amplified exon 5 to 8 of p53 gene was directly sequenced.ResultsH69, which had bcl-2 expression, p53 mutation and decreased expression of p53 and bax, was relatively resistant to cisplatin and delayed and reduced apoptosis. Although apoptosis was markedly reduced in cisplatin-resistant sublines compared to their parental cells, there were no significant differences in the expression of p53, bcl-2 and bax.ConclusionsCisplatin-resistance was associated with the reduced cellular susceptibility to apoptosis. Cancer cells with the natural expression of bcl-2 and p53 mutation may be more resistant to cisplatin and less susceptible to apoptosis.
ISSN	12263303
Volume Number	14
PubMed Central reference number	PMC4531897
Issue Number	1
PubMed reference number	10063313
Journal	The Korean Journal of Internal Medicine [Korean J. Intern. Med]
e-ISSN	20056648
DOI	10.3904/kjim.1999.14.1.42
Language	English
Publisher	Korean Association of Internal Medicine
Publisher Date	1999-01-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 1999 The Korean Association of Internal Medicine
Subject Keyword	Cisplatin-resistance apoptosis p53 bcl-2 bax
Content Type	Text
Resource Type	Article
Subject	Internal Medicine