NDLI: Beneficial effects of dark chocolate on exercise capacity in sedentary subjects: underlying mechanisms. A double blind, randomized, placebo controlled trial

Content Provider	Royal Society of Chemistry (RSC)
Author	Taub, Pam R. Patel, Minal Román-Pintos, Luis Miguel Villarreal, Francisco Ramirez-Sanchez, Israel Higginbotham, Erin Perkins, Guy Phillips, Paul Ceballos, Guillermo Moreno-Ulloa, Aldo
Copyright Year	2016
Abstract	In heart failure patients the consumption of (−)-epicatechin ((−)-Epi)-rich cocoa can restore skeletal muscle (SkM) mitochondrial structure and decrease biomarkers of oxidative stress. However, nothing is known about its effects on exercise capacity and underlying mechanisms in normal, sedentary subjects. Twenty normal, sedentary subjects (∼50 years old) were randomized to placebo or dark chocolate (DC) groups and consumed 20 g of the products for 3 months. Subjects underwent before and after treatment, bicycle ergometry to assess VO2 max and work, SkM biopsy to assess changes in mitochondrial density, function and oxidative stress and blood sampling to assess metabolic endpoints. Seventeen subjects completed the trial. In the DC group (n = 9), VO2 max increased (17% increase, p = 0.056) as well as maximum work (watts) achieved (p = 0.026) with no changes with placebo (n = 8). The DC group evidenced increases in HDL levels (p = 0.005) and decreased triglycerides (p = 0.07). With DC, SkM evidenced significant increases in protein levels for LKB1, AMPK and PGC1α and in their active forms (phosphorylated AMPK and LKB1) as well as in citrate synthase activity while no changes were observed in mitochondrial density. With DC, significant increases in SkM reduced glutathione levels and decreases in protein carbonylation were observed. Improvements in maximum work achieved and VO2 max may be due to DC activation of upstream control systems and enhancement of SkM mitochondria efficiency. Larger clinical studies are warranted to confirm these observations.
Starting Page	3686
Ending Page	3693
Page Count	8
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	7
Issue Number	9
Journal	Food & Function
DOI	10.1039/c6fo00611f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	AMPK Heart failure Catechin Chocolate Skeletal muscle Oxidative stress Placebo Bicycle VO2 max Biopsy High-density lipoprotein Triglyceride Protein STK11 AMP-activated protein kinase Citrate synthase Glutathione Carbonylation Mitochondrion
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Quantitative Proteomic Profiling of Muscle Type-Dependent and Age-Dependent Protein Carbonylation in Rat Skeletal Muscle Mitochondria

1 carbohydrate ingestion does not alter skeletal muscle ampk signaling during exercise in humans.

Quantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondria.

Skeletal muscle mitochondrial respiration in AMPK 2 kinase-dead mice.

Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans

NPC-26 kills human colorectal cancer cells via activating AMPK signaling.

Accumulation of triglyceride-rich lipoprotein in subjects.

Suppression of 5′-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle

Age-induced morphological, biochemical, and functional alterations in isolated mitochondria from murine skeletal muscle.

Beneficial effects of dark chocolate on exercise capacity in sedentary subjects: underlying mechanisms. A double blind, randomized, placebo controlled trial

Similar Documents

Quantitative Proteomic Profiling of Muscle Type-Dependent and Age-Dependent Protein Carbonylation in Rat Skeletal Muscle Mitochondria

1 carbohydrate ingestion does not alter skeletal muscle ampk signaling during exercise in humans.

Quantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondria.

Skeletal muscle mitochondrial respiration in AMPK 2 kinase-dead mice.

Calcineurin Antagonizes AMPK to Regulate Lipolysis in Caenorhabditis elegans

NPC-26 kills human colorectal cancer cells via activating AMPK signaling.

Accumulation of triglyceride-rich lipoprotein in subjects.

Suppression of 5′-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle

Age-induced morphological, biochemical, and functional alterations in isolated mitochondria from murine skeletal muscle.

Beneficial effects of dark chocolate on exercise capacity in sedentary subjects: underlying mechanisms. A double blind, randomized, placebo controlled trial