NDLI: Bio-assay guided identification of hepatoprotective polyphenols from <i>Penthorum chinense</i> Pursh on <i>t</i>-BHP induced oxidative stress injured L02 cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Wang, Anqi Wang, Shengpeng Lin, Ligen Jiang, Yun Wang, Yitao Chen, Meiwan
Copyright Year	2016
Abstract	Penthorum chinense Pursh (Ganhuangcao in Chinese) is traditionally used for liver protection and treatment of liver diseases, including hepatitis B, hepatitis C and alcoholic liver damage. We and others have disclosed the hepatoprotective effect of different extracts from P. chinense. To further explore the chemical principles responsible for its liver protective effect, a bioactivity guided isolation was carried out on the water extract of P. chinense, which led to the identification of an effective fraction (E50M60). Further isolation of the E50M60 fraction produced ten polyphenols. Among them, quercetin showed the most significant protective effect on tert-butyl hydroperoxide (t-BHP) induced hepatocyte damage. Further study demonstrated that both the E50M60 fraction and quercetin attenuated t-BHP induced hepatocyte apoptosis through up-regulating the expression of B-cell lymphoma-2 protein (BCL-2) and BCL-xL, and down-regulating the cleaved products of caspase-7, -9 and nuclear poly(ADP-ribose) polymerase (PARP). Moreover, the E50M60 fraction and quercetin promoted nuclear factor-like 2 (NRF2), superoxide dismutase-2 (SOD-2) and heme oxygenase-1 (HO-1) expressions and suppressed Kelch-like ECH-associated protein 1 (KEAP-1) expression, resulting in resistance to the reactive oxygen species (ROS) induced mitochondrial oxidative stress. Altogether, both the active fraction and quercetin from P. chinense might be well developed as a novel functional food for liver protection.
Starting Page	2074
Ending Page	2083
Page Count	10
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	7
Issue Number	4
Journal	Food & Function
DOI	10.1039/c6fo00110f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Ganhuangcao Penthorum Frederick Traugott Pursh Liver Hepatitis B virus Hepatitis C virus Hepatoprotection Quercetin Tert-Butyl hydroperoxide Hepatocyte Apoptosis B-cell lymphoma Protein Bcl-2 Caspase Poly (ADP-ribose) polymerase Superoxide dismutase Heme oxygenase HMOX1 Reactive oxygen species Oxidative stress Functional food
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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