NDLI: Determination of thiamine (vitamin B<sub>1</sub>) in pharmaceutical tablets and human urine by titania-based ligand-exchange hydrophilic interaction chromatography

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Rong Tan, Jin Jiang, Zi-Tao
Copyright Year	2011
Abstract	A new method for the determination of thiamine (vitamin B1) using high performance liquid chromatography (HPLC) on a titania-based column with UV detection has been developed. The influence of acetonitrile (MeCN) percentage in mobile phase, phosphate buffer concentration and buffer pH on the retention of thiamine were investigated. Thiamine retention on titania was ascribed to the cooperation of hydrophilic interaction and ligand-exchange. By altering the buffer concentration, the retention of thiamine on titania was easily adjusted. Isolation of thiamine in pharmaceutical tablets and human urine was achieved within 10 min. Neither complex pretreatment procedures during the sample preparation nor ion-pair reagents in the eluent were needed to retain thiamine. Hydrophobic or non-titania-affinitive interferences were weakly retained, while both the hydrophilic and titania-affinitive thiamine obtained moderate retention (retention factor k = 1.2). The method presented good linearity (R2 = 0.9997) toward thiamine in the range of 0.25–125 μg mL−1. The limit of detection (3σ) for thiamine was 75 ng mL−1. The precision (RSD) for 11 replicate detections of 5 μg mL−1thiamine was 2.3%. The recovery of spiked thiamine in vitamin B1 tablets, compound vitamin B tablets and human urine ranged from 96 to 114%. The proposed titania-based HPLC method was employed to monitor the time-dependent concentration of thiamine in human urine from a healthy volunteer after oral medication of vitamin B1 tablets. This finding is not only a supplement to the detecting methods for thiamine, but also adds new merits to titania-based stationary phase.
Starting Page	1568
Ending Page	1573
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	7
Journal	Analytical Methods
DOI	10.1039/c1ay05156c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Urine Elution Phosphate Acetonitrile PH Retardation factor Hydrophile High-performance liquid chromatography Vitamin B12 Thiamine Chromatography
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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