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Analytical Methods Vol - 9 Issue - 4

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Chemometric evaluation of hydrophilic interaction liquid chromatography stationary phases: resolving complex mixtures of metabolites

Content Provider	Royal Society of Chemistry (RSC)
Author	Navarro-Reig, Meritxell Jaumot, Joaquim Tauler, Romà Ortiz-Villanueva, Elena
Copyright Year	2017
Abstract	Different hydrophilic interaction liquid chromatography (HILIC) stationary phases have been evaluated using different chemometric methods with the aim of their application in metabolomics studies. Experimental factors, such as the type of HILIC stationary phase (i.e. amide, amine, zwitterionic and diol) and the mobile phase conditions (organic co-solvent, pH and ionic strength) were assessed using a full factorial experimental design. A test sample mixture of metabolites with diverse physicochemical properties (amino acids, nucleotides, nucleosides, and sugars among others) was analyzed by liquid chromatography with a diode array detector (LC-DAD) using five different HILIC columns. Application of multivariate curve resolution alternating least squares (MCR-ALS) method, allowed the full chromatographic peak resolution of all mixture constituents. This approach was particularly helpful in the case of methanol samples where the quality of the chromatographic separation (resolution) was lower in consequence of the co-solvent perturbation on the water layer formation at the surface of the stationary phase. Then, Berridge chromatographic response function (CRF), based on peak resolution, retention times and number of peaks, was used for the investigation of the best HILIC column configuration for future metabolomics studies. The best chromatographic configuration resulted in being the amide and zwitterionic HILIC stationary phases in combination with acetonitrile as organic co-solvent of the mobile phase.
Starting Page	774
Ending Page	785
Page Count	12
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	9
Issue Number	5
Journal	Analytical Methods
DOI	10.1039/c6ay02976k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Diol Chromatography Factorial experiment Chromatographic response function Ionic strength Acetonitrile PH Amide Chromatography detector Hydrophile Berridge Methanol
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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