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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Sharon, Andre Gruentzig, Alexander W. Klapperich, Catherine M. Chatterjee, Anirban Braman, Jeff Sauer-Budge, Alexis F. |
| Copyright Year | 2011 |
| Abstract | Effective DNA purification is essential for reliable composition and contamination analysis in the food industry. This paper compares two DNA extraction and purification procedures for food analysis. The commercially available DNA extraction kit from Agilent Technologies, Inc. was tested against a new solid phase extraction (SPE) method using silica beads embedded in a porous monolith structure that is integrated in a disposable column design. The technique is amenable to automation and streamlined workflow integration, with possible miniaturization of the sample handling process making it suitable for high-throughput applications. To demonstrate the automated process, the manual homogenizing step was compared to a custom automated procedure. To demonstrate the process for food testing, two types of sausages were analyzed for traces of porcine gDNA. |
| Starting Page | 1507 |
| Ending Page | 1513 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 17599660 |
| Volume Number | 3 |
| Issue Number | 7 |
| Journal | Analytical Methods |
| DOI | 10.1039/c0ay00701c |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Solid phase extraction DNA extraction Automation Agilent Technologies DNA Monolith The Technique Silicon dioxide |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Engineering Chemical Engineering |
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