NDLI: A immunosensor for the diagnosis of canine distemper virus infection using SPR and EIS

Content Provider	Royal Society of Chemistry (RSC)
Author	Ribeiro, Marcela Cristina Mendes Tozato, Claudia de Camargo Junior, João Pessoa Araujo Pedrosa, Valber A. Basso, Caroline Rodrigues
Copyright Year	2013
Abstract	Canine distemper virus (CDV) is a viral disease that affects dogs and many other carnivores. Clinical diagnosis of CDV is difficult due to the broad spectrum of signs that may be confounded with other respiratory and enteric diseases of dogs. Laboratory analysis is required to diagnose suspected cases. In this study, surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) methodologies were developed for the detection of canine distemper virus simultaneously. The assay exhibited high specificity, as all the negative controls were not mistakenly detected. The CDV concentration was determined from successive injections into the apparatus, with a linear range from 1.1 to 116.0 ng mL−1. The system exhibited good reproducibility with 4.5% variation between runs after regeneration of the coated surface with a solution of 0.1 M glycine–HCL (pH 3.0). The capacitance and resistance values of the modified interface were calculated from EIS data using an equivalent circuit. It was possible to measure CDV in highly concentrated viruses with good specificity and reproducibility.
Starting Page	5089
Ending Page	5095
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	5
Issue Number	19
Journal	Analytical Methods
DOI	10.1039/c3ay41087k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	HCL Virus Spectroscopy Electrochemistry PH Capacitance Surface plasmon resonance Surface plasmon EIS Canine distemper Electrical impedance
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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