NDLI: Detection of Canine Distemper Virus by Surface Plasmon Resonance

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Detection of Canine Distemper Virus by Surface Plasmon Resonance

Content Provider	Semantic Scholar
Author	Basso, Caroline Rodrigues Sempionatto, Juliane R. Tozato, Cláudia Araújo, João Pessoa Perez, Geraldo Gabriel
Copyright Year	2015
Abstract	A PMMA polymer adsorbed in gold sensor through surface plasmon resonance (SPR) technique was developed for detection the canine distemper virus (CDV) through antibody/antigen binding. The antibodies anti-CDV were physically adsorbed on the surface of the sensor through covalent bonds with the thiol group formed by PMMA/EDC-NHS, the urine samples with the CDV virus were deposited on the sensor to be detected. The highest sensitive sensor surface for urine sample detection was the 200μg/ml of immobilized in 20μg/ml of antibodies, with 1 μg/ml of detection limit yielding 50 millidegree of SPR angle shift. After each binding experiment, the sensor surface was completely regenerated prior to the next measurement using 0.2 mol L −1 glycine–HCl (pH 3). All assays were performed in triplicate showing a high efficiency and good reproducibility of the technique.
Starting Page	1483
Ending Page	1490
Page Count	8
File Format	PDF HTM / HTML
DOI	10.5151/chemeng-cobeq2014-1074-21218-141890
Volume Number	1
Alternate Webpage(s)	http://pdf.blucher.com.br.s3-sa-east-1.amazonaws.com/chemicalengineeringproceedings/cobeq2014/1074-21218-141890.pdf
Alternate Webpage(s)	https://doi.org/10.5151/chemeng-cobeq2014-1074-21218-141890
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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