NDLI: Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA

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Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA

Content Provider	Royal Society of Chemistry (RSC)
Author	Ma, Xiaoming Zheng, Hanye Chen, Lisong Qiu, Bin Lin, Zhenyu Chen, Guonan Guo, Longhua
Copyright Year	2013
Abstract	A simple and label-free DNA electrochemical impedance biosensor has been developed for the sequence-specific recognition of double-stranded DNA (ds-DNA). The probe triplex-forming oligonucleotide (TFO) is immobilized on the gold electrode through Au–S interaction. In the presence of the target ds-DNA, the probe TFO interacted with the bases in the major groove of the ds-DNA to form a rigid triplex structure via Hoogsteen hydrogen bonds, leading to an increase of the electron-transfer resistance. It is found that the change of impedance has a linear relationship with the concentration of the target in the range of 0.1–40 nmol L−1, and the detection limit is as low as 0.04 nmol L−1 (S/N = 3).
Starting Page	5005
Ending Page	5009
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	5
Issue Number	19
Journal	Analytical Methods
DOI	10.1039/c3ay40972d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Electrode DNA Biosensor Hoogsteen base pair Electrochemistry Oligonucleotide TFO Hoogsteen Electrical impedance Hydrogen bond
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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