NDLI: Determination of cyanuric acid in whey powder using HILIC-MS/MS

Content Provider	Royal Society of Chemistry (RSC)
Author	Babić, Sandra Ivešić, Martina Krivohlavek, Adela Šmit, Zdenko
Copyright Year	2013
Abstract	A hydrophilic liquid chromatography-tandem mass spectrometry method was developed for determination of cyanuric acid in whey powder samples in the mass fraction range from 1.00 to 100.0 mg kg−1. Cyanuric acid was extracted from whey powder with an acetonitrile–water (60 : 40, v/v) extraction solution. Separation was performed on a 3.5 μm XBridge Amide column using acetonitrile–water (90 : 10, v/v) as the mobile phase at a flow rate of 0.2 mL min−1. Detection was achieved by triple quadrupole mass spectrometry using a heated electrospray ionization interface. The analysis was performed in the negative ionization mode using the SRM transition combinations of m/z 128 → 42 and m/z 128 → 85 for quantitative and qualitative detection of cyanuric acid, respectively, and m/z 134 → 44 and m/z 134 → 89 for isotope-labelled cyanuric acid (13C3, 15N3-CYA) detection, used to correct for matrix effects. The developed and optimized method was validated by determining the following validation parameters: selectivity, linearity, precision, accuracy, recovery, limits of detection and quantification and stability of cyanuric acid in whey powder extracts. The recoveries of cyanuric acid from whey powder spiked at 1.00, 2.50, 7.00, 50.0 and 100.0 mg kg−1 were within the range of 96.7–107.2%, intra-day precision was ≤6.45%, inter-day precision was ≤9.13% and accuracy was below 10%. Matrix effects investigated with three different matrices were less than 15%. The limits of detection and quantification were 0.30 mg kg−1 and 1.00 mg kg−1, respectively. The suitability of the developed method for use in routine determination was confirmed through analysis of whey powder samples from dairy industry and supermarkets.
Starting Page	5188
Ending Page	5194
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	5
Issue Number	19
Journal	Analytical Methods
DOI	10.1039/c3ay40632f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Quadrupole mass analyzer Acid Ionization Electrospray ionization Micrometre Positive-definite matrix Amide Hydrophile Whey Propellant mass fraction Mass spectrometry SRM
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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